DEVICE AND METHOD FOR ISOLATING AND CULTIVATING LIVE CELLS ON A FILTER OR EXTRACTING THE GENETIC MATERIAL THEREOF

• US 20110070642A1
• Filed: 01/08/2009
• Published: 03/24/2011
• Est. Priority Date: 01/09/2008
• Status: Active Grant

• Current Assignee: ScreenCell, Co.
• Original Assignee: ScreenCell, Co.
• Inventors: Cayre, Yvon
• Granted Patent: US 9,339,818 B2
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/5635 connecting two containers face to face, e.g. comprising a filter
• CPC Class Codes: C12M 33/14 with filters, sieves or membranes
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12N 15/1017 by filtration, e.g. using filters, frits, membranes

Abstract

A device for isolating and cultivating live cells on a filter or for extracting the genetic material thereof includes: a filter holder (108) connected to a filter; a compartment (102) having an upper opening and a lower opening; and an element (110) that is mobile relative to the compartment for applying a force on the holder and releasing the holder. According to the embodiments, the filter holder is mechanically connected to the compartment or to the mobile element until the application of the force. Preferably, the device further includes a removable end piece (104) tightly and removably attached and adapted for preventing the relative movement of the mobile element and the compartment for applying the force and releasing the holder.

Claims

1. Device for isolating live cells on a filter, characterized in that it includes:

a filter support (108, 308) fastened to a filter,

a compartment (102, 302) having an upper opening and a lower opening, and

means (110, 310) mobile relative to said compartment for applying a force to the support and releasing said support.

2. Device according to claim 1, characterized in that it includes a removable end-piece (104, 304) removably fixed and sealed and adapted to prohibit relative movement of the mobile means (110, 310) and said compartment (102, 302) that makes it possible to apply said force and release said filter support (108, 308).

3. Device according to claim 2, characterized in that said end-piece (104, 304) has a lower opening that fits onto a reduced-pressure chamber of an aspiration system.

4. Device according to claim 1, characterized in that said filter support (108) takes the form of a washer.

5. Device according to claim 4, characterized in that said filter support (108) is in PVC.

6. Device according to claim 5, characterized in that said filter support (108) is in rigid PVC.

7. Device according to claim 1, characterized in that said filter support (108) takes the form of an object cover slide.

8. Device according to claim 1, characterized in that said filter support (108) has dimensions greater than or equal to those of an object cover slide.

9. Device according to claim 1, characterized in that said filter support (108) has a thickness less than or equal to 0.4 mm and preferably 0.3 mm.

10. Device according to claim 1, characterized in that said filter support (308) takes the form of at least part of an Eppendorf tube (330).

11. Device according to claim 10, characterized in that the interior upper part of said filter support (308) reproduces the shape of the interior upper part of an Eppendorf tube (330).

12. Device according to claim 10, characterized in that the interior lower part of said filter support (308) reproduces the shape of the interior lower part of an Eppendorf tube (330).

13. Device according to claim 10, characterized in that the exterior upper part of said filter support (308) reproduces the shape of the interior upper part of an Eppendorf tube (330).

14. Device according to claim 10, characterized in that the filter support (308) includes polycarbonate.

15. Device according to claim 1, characterized in that the filter support (108, 308) is mechanically connected to said compartment (102, 302) until application of said force.

16. Device according to claim 1, characterized in that said filter support (108, 308) is mechanically connected to said mobile means until application of said force.

19. Device according to claim 11, characterized in that the interior lower part of said filter support (308) reproduces the shape of the interior lower part of an Eppendorf tube (330).

17. Method for isolating live cells on a filter or extracting their genetic material, characterized in that it includes:

a step of fastening a filter support fastened to a filter to a compartment having an upper opening and a lower opening or to means mobile relative to said compartment, and

a step of moving said mobile means and said compartment to apply a force to the filter support and release said filter support.

18. Method according to claim 17, characterized in that, during the movement step, the filter support (108) is moved directly to a culture well (130).