FULLY HUMAN ANTI-VEGF ANTIBODIES AND METHODS OF USING
First Claim
Patent Images
1. An isolated antibody or antigen-binding fragment thereof comprising,a heavy chain variable region which comprises the following heavy chain CDR amino acid sequences:
- SEQ ID NO;
6, SEQ ID NO;
7 and SEQ ID NO;
8, and/or2) a light chain variable region which comprises the following light chain CDR amino acid sequences (a) SEQ ID NO;
9, SEQ ID NO;
10 and SEQ ID NO;
11, or (b) SEQ ID NO;
12, SEQ ID NO;
13 and SEQ ID NO;
14.
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0 Petitions
Accused Products
Abstract
Vascular endothelial growth factor (VEGF) overexpression is associated with a variety of conditions involving aberrant angiogenesis. Disclosed herein are fully human antibodies that specifically bind human VEGF and inhibit VEGF binding to VEGF-R1 and VEGF-R2, and therefore inhibit VEGF signaling Based on their ability to inhibit VEGF signaling, the antibodies disclosed herein may be used to treat angiogenesis and conditions associated with angiogenesis both in vivo and in vitro.
26 Citations
48 Claims
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1. An isolated antibody or antigen-binding fragment thereof comprising,a heavy chain variable region which comprises the following heavy chain CDR amino acid sequences:
- SEQ ID NO;
6, SEQ ID NO;
7 and SEQ ID NO;
8, and/or2) a light chain variable region which comprises the following light chain CDR amino acid sequences (a) SEQ ID NO;
9, SEQ ID NO;
10 and SEQ ID NO;
11, or (b) SEQ ID NO;
12, SEQ ID NO;
13 and SEQ ID NO;
14. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 33, 34, 35, 36, 37, 38, 39, 40, 45)
- SEQ ID NO;
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19. An isolated polynucleotide encoding the amino acid sequence set forth in any of SEQ ID NOs:
- 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or 14.
- View Dependent Claims (20, 21)
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22. A fully human antibody wherein the antibody comprises one or more characteristics selected from the group consisting of:
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(1) binding to hVEGF165 with a KD no less than 10-fold lower than the KD with which the antibody binds mVEGF165;
(2) binding to hVEGF165 with a KD of ≦
2.0 nM;(3) binding to an epitope on hVEGF165 that overlaps with the epitope bound by Bevacizumab; (4) blocking binding of hVEGF165 to a VEGF receptor;
or(5) inhibiting hVEGF165-induced phosphorylation of a VEGF receptor. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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41. An isolated human antibody comprising a light chain variable region comprising the amino acid sequence set forth in SEQ ID NO:
- 3 and a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO;
2. - View Dependent Claims (43)
- 3 and a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO;
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42. An isolated human antibody comprising a light chain variable region comprising the amino acid sequence set forth in SEQ ID NO:
- 5 and a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO;
4. - View Dependent Claims (44)
- 5 and a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO;
- 46. An isolated antibody or an antigen fragment thereof comprising at least one heavy chain complementarity determining region of XPA.10.064 or XPA.10.072 and at least one light chain complementarity determining region of XPA.10.064 or XPA.10.072.
Specification