PHOTON REDUCING AGENTS FOR FLUORESCENCE ASSAYS
First Claim
1. A method of reducing undesired light emission from a sample, comprising:
- contacting a sample in need of reducing undesired light with at least one photon reducing agent, wherein said sample comprises a membrane compartment in contact with a solid surface, wherein said membrane compartment includes at least one photon producing agent and said at least one photon reducing agent is in an aqueous solution that contacts an outer surface of said membrane compartment, anddetecting an optical signal from said at least one photon producing agent.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
56 Citations
81 Claims
-
1. A method of reducing undesired light emission from a sample, comprising:
-
contacting a sample in need of reducing undesired light with at least one photon reducing agent, wherein said sample comprises a membrane compartment in contact with a solid surface, wherein said membrane compartment includes at least one photon producing agent and said at least one photon reducing agent is in an aqueous solution that contacts an outer surface of said membrane compartment, and detecting an optical signal from said at least one photon producing agent. - View Dependent Claims (2, 3, 5, 6, 8, 10, 11, 18, 19, 20, 21, 23, 80)
-
-
4. (canceled)
-
7. (canceled)
-
9. (canceled)
-
12-17. -17. (canceled)
-
22. (canceled)
-
24-48. -48. (canceled)
-
49. A composition of matter, comprising:
-
a) a membrane compartment in contact with a solid surface, wherein said membrane compartment comprises at least one photon producing agent, and b) an aqueous solution with at least one photon reducing agent, wherein said aqueous solution in contact with an outer surface of said membrane compartment. - View Dependent Claims (56, 60, 67)
-
-
50-55. -55. (canceled)
-
57-59. -59. (canceled)
-
61-66. -66. (canceled)
-
68-70. -70. (canceled)
-
71. A method of identifying a chemical with a biological activity, comprising:
-
a) contacting a sample with a test chemical, said sample comprising a target, b) contacting said sample with at least one photon reducing agent, wherein said sample comprises a membrane compartment in contact with a solid surface, wherein said membrane compartment includes at least one photon producing agent that directly or indirectly monitors the activity of said target and said at least one photon reducing agent is in an aqueous solution that contacts the outer surface of said membrane compartment, and c) detecting an optical signal from said at least one photon producing agent, wherein said at least one photon reducing agent has an absorption spectra that overlaps with the absorption, emission or excitation spectrum of said at least one photon producing agent or wherein said at least one photon producing agent can exhibit fluorescence resonance energy transfer with said at least one photon reducing agent. - View Dependent Claims (81)
-
-
72-79. -79. (canceled)
Specification