MAGNETICALLY-DRIVEN BIODEGRADABLE GENE DELIVERY NANOPARTICLES FORMULATED WITH SURFACE-ATTACHED POLYCATIONIC COMPLEX
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Abstract
A particle including a matrix-forming agent and a polyelectrolyte-amphiphilic agent adduct wherein the polyelectrolyte-amphiphilic agent adduct is in physical communication with the matrix-forming agent. The particle further includes a coated magnetic field-responsive agent and a biomaterial. Methods of making the particle are provided. Also provided are methods of delivery of the biomaterial to a target cell or a target tissue including administering the particle having the matrix-forming agent, polyelectrolyte-amphiphilic agent adduct, the coated magnetic field-responsive agent and the biomaterial; providing a magnetic device associated with the target cell or the target tissue; applying a magnetic force to the particle; and guiding the particle toward the magnetic device by the magnetic force.
59 Citations
90 Claims
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1-53. -53. (canceled)
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54. A particle comprising:
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a matrix-forming agent; and a polyelectrolyte-amphiphilic agent complex formed by an ionic association of a polyelectrolyte with a first amphiphilic agent and having a C4-C24 hydrocarbon chain, wherein the polyelectrolyte-amphiphilic agent complex is in physical communication with the matrix-forming agent. - View Dependent Claims (55, 56, 57, 58, 59, 60, 61, 62, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81)
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63. A magnetic particle, the magnetic particle comprising:
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a matrix-forming polymer; and a coated magnetic field-responsive agent comprising a magnetic field-responsive agent and an amphiphilic agent, wherein the coated magnetic field-responsive agent is in communication with the matrix-forming polymer, provided that the magnetic particle is free of a polyelectrolyte. - View Dependent Claims (64, 82, 83, 84, 85)
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86. A method of making a particle, the method comprising:
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providing a first medium and a second medium; providing a coated magnetic field-responsive agent; optionally providing a stabilizer; providing a composition comprising a matrix-forming agent, a polyelectrolyte, a first amphiphilic agent and optionally the stabilizer; dispersing the coated magnetic field-responsive agent in the first medium to form a dispersion; mixing the composition with the dispersion; adding the second medium to the composition and the dispersion to form a first mixture; emulsifying the first mixture to give a first emulsion; and removing the first medium and thereby forming the particle. - View Dependent Claims (87, 88)
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89. A method of delivery of a biomaterial to a target cell or a target tissue, the method comprising:
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administering a magnetic particle comprising (i) a matrix-forming polymer;
(ii) a coated magnetic field-responsive agent comprising a magnetic field-responsive agent and a second amphiphilic agent, wherein the coated magnetic field-responsive agent is in communication with the matrix-forming polymer, provided that the magnetic particle is free of a polyelectrolyte; and
(iii) the biomaterial in communication with the matrix-forming polymer;optionally providing a magnetic device associated with the target cell or the target tissue; applying a magnetic force to the particle; and guiding the particle by the magnetic force and thereby delivering the biomaterial to the target cell or the target tissue.
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90. A method of delivery of a biomaterial to a cell or a tissue, the method comprising:
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administering a particle comprising (i) a matrix-forming agent;
(ii) a polyelectrolyte-amphiphilic agent complex wherein the polyelectrolyte-amphiphilic agent complex is in physical communication with the matrix-forming agent; and
(iii) a biomaterial in communication with at least one of the polyelectrolyte-amphiphilic agent complex or the matrix-forming agent, wherein the particle is free of the magnetic field-responsive agent; anddelivering the biomaterial to the cell or tissue using the particle as a carrier, wherein the cell is optionally contacted with a transfection agent prior to said delivering.
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Specification