METHOD AND CELL FOR MEASURING THE GLOBAL ION CONCENTRATION OF A BODY FLUID

• US 20110079521A1
• Filed: 09/20/2010
• Published: 04/07/2011
• Est. Priority Date: 10/02/2009
• Status: Active Grant

• Current Assignee: Commissariat a L'Energie Atomique (French Alternative Energies and Atomic Energy Commission)
• Original Assignee: Commissariat a L'Energie Atomique (French Alternative Energies and Atomic Energy Commission)
• Inventors: REVOL-CAVALIER, Frédéric
• Granted Patent: US 8,354,017 B2
• Time in Patent Office: Days
• US Class Current: 205/789
• CPC Class Codes: A61B 10/0064 for taking sweat or sebum samples measuring sweat production A61B5/4266
• CPC Class Codes: A61B 5/4266 sweat secretion
• CPC Class Codes: A61B 5/4277 saliva secretion
• CPC Class Codes: G01N 27/07 Construction of measuring vessels; Electrodes therefor

Abstract

Method for measuring the global ion concentration of a body fluid that comprises application of a stable DC voltage between first and second electrodes of a cell for measuring the global ion concentration of a body fluid so as to cause electrochemical hydrolysis reactions of the body fluid water to occur at the level of said first and second electrodes, measurement of a hydrolysis current generated by said electrochemical hydrolysis reactions of the water and determination of the global ion concentration of the body fluid by comparison with a previously defined calibration curve. The measuring cell comprises a fluid channel having an internal cross-section smaller than or equal to 1.5 mm.

Claims

1. A method for measuring the global ion concentration of a body fluid comprising the following steps:

application of a stable DC voltage between first and second electrodes of a cell for measuring the global ion concentration of a body fluid so as to cause electrochemical hydrolysis reactions of the body fluid water to occur at the level of said first and second electrodes,

measurement of a hydrolysis current generated by said electrochemical hydrolysis reactions of water and,

determination of the global ion concentration of the body fluid by comparison with a previously defined calibration curve,

said measuring cell comprising a fluid channel arranging at least one passage enabling the body fluid to flow from an inlet to an outlet, said fluid channel having an internal cross-section less than or equal to 1.5 mm between the first and second electrodes arranged on said passage, so that the ion concentration of the body fluid is the factor limiting the kinetics of said electrochemical hydrolysis reactions of the water.

2. The method according to claim 1, wherein measurement of the hydrolysis current is performed by chronoamperometry during application of the DC voltage.

3. The method according to claim 1, wherein the measuring cell comprises a constriction of the internal cross-section of the fluid channel between the first and second electrodes.

4. The method according to claim 1, wherein the voltage applied is comprised between 2V and 10V.

5. The method according to claim 1, comprising an immersion step of the measuring cell in the body fluid before the voltage is applied.

17. The method according to claim 1, wherein the voltage applied is comprised between 3V and 5V.

6. A cell for measuring the global ion concentration of a body fluid which comprises:

a fluid channel with an inlet and an outlet of the body fluid, and

first and second electrodes connected to a stable DC voltage generator and to an electronic measurement circuit,

said fluid channel comprising first and second zones each having a defined internal cross-section and a third zone situated between the first zone and the second zone and adjacent to said first and second zones and arranging at least one passage for the body fluid,

said third zone having a reduced internal cross-section, smaller than the respective internal cross-sections of said first and second zones, to limit ionic migration between said electrodes in the body fluid,

said first and second electrodes being arranged on said passage and being respectively situated at the level of the first and second zones.

7. The measuring cell according to claim 6, wherein the fluid channel is delineated by a cover and by at least a part of a support, said cover being arranged on the support.

8. The measuring cell according to claim 7, wherein the support comprises means for draining the body fluid to the fluid channel.

9. The measuring cell according to claim 7, wherein the cover comprises at least one drain hole passing through the wall of the cover and situated outside the third zone.

10. The measuring cell according to claim 7, comprising a body fluid collecting device and wherein the support of fluid channel forms at least a part of said collecting device.

11. The measuring cell according to claim 6, wherein the fluid channel comprises at least one constriction at the level of the third zone making the passage narrower.

12. The measuring cell according to claim 6, wherein the first or second electrodes are both formed by a wire passing through the fluid channel.

13. The measuring cell according to claim 6, wherein the first and second electrodes are formed in the wall of the fluid channel.

14. The measuring cell according to claim 6, wherein the first and second electrodes are deposited on one and the same wall of the fluid channel.

15. The measuring cell according to claim 6, comprising a body fluid collecting device.

16. The measuring cell according to claim 6, comprising an additional zone adjacent to the first zone, situated between the inlet and the first zone, and/or an additional zone adjacent to the second zone and situated between the second zone and the outlet, each additional zone having a reduced internal cross-section, smaller than the respective internal cross-sections of said first and second zones.