Water-Soluble Rhodamine Dyes Conjugates
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Abstract
The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
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Citations
66 Claims
- 1. (canceled)
- 2. A rhodamine dye or a salt thereof, comprising a rhodamine-type parent xanthene ring having attached to the xanthene C9 carbon a phenyl group that is further substituted with an ortho carboxy or ortho sulfonate group or a salt thereof, one to three substituted or unsubstituted aminopyridinium groups and a substituted or unsubstituted alkylthio, arylthio or heteroarylthio group, wherein the rhodamine dye comprises the structure:
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3. (canceled)
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13-14. -14. (canceled)
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47-60. -60. (canceled)
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66-69. -69. (canceled)
Specification