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REDUCED CODON MUTAGENESIS

  • US 20110082055A1
  • Filed: 09/17/2010
  • Published: 04/07/2011
  • Est. Priority Date: 09/18/2009
  • Status: Abandoned Application
First Claim
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1. A method of making a library of polynucleotide variants, the method comprising:

  • providing a reference polynucleotide molecule or sequence;

    selecting a plurality of codon sites in the reference polynucleotide molecule or sequence to be varied;

    selecting a degenerate codon set for the plurality of codon sites to be varied, the degenerate codon set comprising a plurality of degenerate codons;

    separately producing a plurality of sets of variant polynucleotide molecules, each variant polynucleotide comprising a member of the degenerate codon set at each of the plurality of sites of codon variation, wherein the set of variant polynucleotide molecules collectively comprises all of the members of the degenerate codon set at each of the sites of codon variation;

    optionally pooling the sets of polynucleotide variants; and

    ,transforming the variants into a population of host cells, thereby producing the library.

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