METHOD FOR FORMING ARTIFICIAL LIPID MEMBRANE

• US 20110083793A1
• Filed: 12/17/2010
• Published: 04/14/2011
• Est. Priority Date: 10/07/2009
• Status: Active Grant

• Current Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Tsukahara, Norihito, Suzuki, Masato, OKI, Akio, Oka, Hiroaki
• Granted Patent: US 8,062,489 B2
• Time in Patent Office: Days
• US Class Current: 156/73.1
• CPC Class Codes: G01N 33/48728 Investigating individual cells, e.g. by patch clamp, voltage clamp investigating individual particles in general G01N15/10

Abstract

An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution.

The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 μl. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; adding a lipid solution to the artificial lipid membrane forming portion; adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; and forming the artificial lipid membrane.

Claims

1. A method for forming an artificial lipid membrane, comprising the steps of:

(A) preparing an artificial lipid membrane forming apparatus comprising a first chamber, a second chamber, a dividing wall sandwiched between the first chamber and the second chamber, and an artificial lipid membrane forming portion consisting of a through hole formed on the dividing wall, the first chamber having a capacity of not smaller than 10 pl and not larger than 200 μ

l, the second chamber having a capacity of not smaller than 10 pl and not larger than 200 μ

l;

(B) adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·

s and not higher than 200 mPa·

s;

(C) adding to the artificial lipid membrane forming portion a lipid solution containing a lipid and an organic solvent;

(D) adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·

s and not higher than 200 mPa·

s to sandwich the lipid solution between the first electrolytic solution and the second electrolytic solution; and

(E) removing the organic solvent to form an artificial lipid membrane at the artificial lipid membrane forming portion.

2. The method according to claim 1, wherein at least one of the first electrolytic solution and the second electrolytic solution contains an organic compound having a hydroxyl group.

3. The method according to claim 2, wherein the organic compound having the hydroxyl group is an alcohol.

4. The method according to claim 3, wherein the alcohol is a lower alcohol.

5. The method according to claim 3, wherein the alcohol is glycerin.

6. The method according to claim 1, wherein at least one of the first electrolytic solution and the second electrolytic solution contains a polymer.

7. The method according to claim 6, wherein at least one of the first electrolytic solution and the second electrolytic solution contains a polyvinyl alcohol.

8. The method according to claim 1, wherein in Step (B), the first electrolytic solution is added to the first chamber by an ink-jet method.

9. The method according to claim 1, wherein in Step (D), the second electrolytic solution is added to the second chamber by an ink-jet method.

10. The method according to claim 1, wherein in Step (C), the lipid solution is added to the artificial lipid membrane forming portion by an ink-jet method.

11. The method according to claim 1, further comprising the step of:

(F), implanting at least one of a receptor and an ion channel in the artificial lipid membrane after Step (E).

12. The method according to claim 1, wherein in Step (B), the first chamber is filled with the first electrolytic solution.

13. The method according to claim 1, wherein in Step (D), the second chamber is filled with the second electrolytic solution.

14. The method according to claim 12, wherein in Step (D), the second chamber is filled with the second electrolytic solution.