METHOD FOR FORMING ARTIFICIAL LIPID MEMBRANE
First Claim
1. A method for forming an artificial lipid membrane, comprising the steps of:
- (A) preparing an artificial lipid membrane forming apparatus comprising a first chamber, a second chamber, a dividing wall sandwiched between the first chamber and the second chamber, and an artificial lipid membrane forming portion consisting of a through hole formed on the dividing wall, the first chamber having a capacity of not smaller than 10 pl and not larger than 200 μ
l, the second chamber having a capacity of not smaller than 10 pl and not larger than 200 μ
l;
(B) adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·
s and not higher than 200 mPa·
s;
(C) adding to the artificial lipid membrane forming portion a lipid solution containing a lipid and an organic solvent;
(D) adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·
s and not higher than 200 mPa·
s to sandwich the lipid solution between the first electrolytic solution and the second electrolytic solution; and
(E) removing the organic solvent to form an artificial lipid membrane at the artificial lipid membrane forming portion.
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Accused Products
Abstract
An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution.
The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 μl. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; adding a lipid solution to the artificial lipid membrane forming portion; adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; and forming the artificial lipid membrane.
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Citations
14 Claims
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1. A method for forming an artificial lipid membrane, comprising the steps of:
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(A) preparing an artificial lipid membrane forming apparatus comprising a first chamber, a second chamber, a dividing wall sandwiched between the first chamber and the second chamber, and an artificial lipid membrane forming portion consisting of a through hole formed on the dividing wall, the first chamber having a capacity of not smaller than 10 pl and not larger than 200 μ
l, the second chamber having a capacity of not smaller than 10 pl and not larger than 200 μ
l;(B) adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·
s and not higher than 200 mPa·
s;(C) adding to the artificial lipid membrane forming portion a lipid solution containing a lipid and an organic solvent; (D) adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·
s and not higher than 200 mPa·
s to sandwich the lipid solution between the first electrolytic solution and the second electrolytic solution; and(E) removing the organic solvent to form an artificial lipid membrane at the artificial lipid membrane forming portion. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification