Method and apparatus to conduct kinetic analysis of platelet function in whole blood samples
First Claim
1. An apparatus for the monitoring or assay of blood platelet adhesion or aggregation, the apparatus including a sample flow chamber, a blood flow inlet providing fluid communication into the flow chamber and a blood flow outlet providing fluid communication from said flow chamber,said flow chamber being defined in part by a plurality of sidewalls, and at least one optically transparent wall portion,the at least one said optically transparent wall portion having a chemically inert substrate having a polydimethylsiloxane PDMS compound coated thereon.
1 Assignment
0 Petitions
Accused Products
Abstract
Adhesion of platelets to blood vessel walls is the first step that promotes arrest of bleeding by interaction of the platelet receptors with various extracellular matrix proteins that become exposed on vascular injury. A flow chamber is provided for use in analyzing or studying platelet function, in whole blood, either as part of a batch process or in real time. In the flow chambers, an inert polydimethylsiloxane (PDMS) surface is plasma-activated and a homobifunctional cross-linker is used to immobilize platelet-binding proteins onto a chamber wall surface. Immobilized collagen and fibrinogen may thus be assayed by continuously monitoring the adhesion of ADP and Ca2+ activated platelets from a subject, such as a patient having normal or type 2 diabetes (T2D). The flow chamber provides a simplified and robust method for the construction flow chambers which enable the kinetic monitoring of platelet adhesion in whole blood.
-
Citations
17 Claims
-
1. An apparatus for the monitoring or assay of blood platelet adhesion or aggregation, the apparatus including a sample flow chamber, a blood flow inlet providing fluid communication into the flow chamber and a blood flow outlet providing fluid communication from said flow chamber,
said flow chamber being defined in part by a plurality of sidewalls, and at least one optically transparent wall portion, the at least one said optically transparent wall portion having a chemically inert substrate having a polydimethylsiloxane PDMS compound coated thereon.
Specification