CHOLESTEROL SENSOR
First Claim
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1. An electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a second series of reagents comprising(a2) a surfactant comprising one or more bile acid derivatives or salts thereof;
- (b2) a triglyceride hydrolysing reagent;
(c2) glycerol dehydrogenase;
(d2) a coenzyme; and
(e2) a redox agent capable of being oxidised or reduced to form a product;
and electrochemically detecting the amount of product formed.
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Abstract
A method for the determination of the amount of cholesterol in a sample is provided. The method typically provides a breakdown of the HDL and LDL cholesterol contents of the sample.
29 Citations
20 Claims
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1. An electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a second series of reagents comprising
(a2) a surfactant comprising one or more bile acid derivatives or salts thereof; -
(b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product; and electrochemically detecting the amount of product formed. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A reagent mixture for use in an electrochemical method for the determination of the amount of triglyceride in a sample, the reagent mixture comprising
(a2) a surfactant comprising one or more bile acid derivatives or salts thereof; -
(b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product. - View Dependent Claims (12, 13, 14, 15)
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16. A kit for measuring the triglyceride content of a sample, the kit comprising
one or more electrochemical cells, each cell having a working electrode, a reference or pseudo reference electrode and optionally a separate counter electrode; -
a second series of reagents comprising; (a2) a surfactant comprising one or more bile acid derivatives or salts thereof; (b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product, said second series of reagents being associated with one of said electrochemical cells; a power supply for applying a potential across each cell; and a measuring instrument for measuring the resulting electrochemical response of each cell. - View Dependent Claims (17, 18)
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19. A method of breaking down the lipoprotein structure of a sample comprising lipoproteins by using one or more bile acid derivatives or salts thereof as a surfactant, in an electrochemical method for the determination of the amount of triglyceride in a sample, said method comprising reacting the sample with a second series of reagents comprising
(a2) said one or more bile acid derivatives or salts thereof; -
(b2) a triglyceride hydrolysing reagent; (c2) glycerol dehydrogenase; (d2) a coenzyme; and (e2) a redox agent capable of being oxidised or reduced to form a product; and electrochemically detecting the amount of product formed.
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20. A method comprising:
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providing a liquid sample containing an amount of triglycerides, said triglycerides being bound to lipoproteins; freeing the triglycerides from the lipoproteins within the liquid sample by contacting the triglycerides with a surfactant comprising bile acid derivatives or salts thereof configured to break the lipoproteins from the triglycerides; breaking down the freed triglycerides produced within the liquid sample into a proportionate amount of glycerol by reacting the freed triglycerides with a triglyceride hydrolyzing reagent; and electrochemically determining the amount of glycerol in the liquid sample by; reacting the glycerol with a suitable enzyme or enzyme-coenzyme reagent in the presence of a redox mediator; applying an electrical potential to the liquid sample; measuring the resulting electrochemical response; and correlating the measured response to the amount of glycerol; wherein the method provides an indication of the total triglyceride content of the liquid sample.
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Specification