FLOW CYTOMETER APPARATUS FOR THREE DIMENSIONAL DIFRACTION IMAGING AND RELATED METHODS

• US 20110090500A1
• Filed: 06/11/2009
• Published: 04/21/2011
• Est. Priority Date: 06/12/2008
• Status: Active Grant

• Current Assignee: Xin-Hua Hu
• Original Assignee: East Carolina University
• Inventors: Lu, Jun O., Hu, Xin-Hua, Jacobs, Kenneth M.
• Granted Patent: US 9,013,692 B2
• Time in Patent Office: Days
• US Class Current: 356/337
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/0222 from dynamic light scattering, e.g. photon correlation spectroscopy
• CPC Class Codes: G01N 2015/025 Methods for single or grouped particles
• CPC Class Codes: G01N 2015/1497 Particle shape

Abstract

A flow cytometer assembly includes a fluid controller configured to form a hydrodynamically focused flow stream including an outer sheath fluid and an inner core fluid. A coherent light source is configured to illuminate a particle in the inner core fluid. A detector is configured to detect a spatially coherent distribution of elastically scattered light from the particle excited by the coherent light source. An analyzing module configured to extract a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light.

Claims