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Methods of Affinity Maturing Antibodies

  • US 20110092372A1
  • Filed: 10/14/2010
  • Published: 04/21/2011
  • Est. Priority Date: 10/14/2009
  • Status: Active Grant
First Claim
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1. A method of affinity maturing an antibody, comprising the steps of:

  • a. obtaining an amino acid sequence of the antibody light chain variable region (VL) or the antibody heavy chain variable region (VH);

    b. identifying rSDRU residues in the amino acid sequence of the antibody VL or VH;

    c. selecting a subset of the rSDRU residues to be diversified;

    d. selecting a set of amino acids or a universal set of amino acids used for diversifying the subset of the rSDRU residues;

    e. preparing a library of antibody VL or VH variants by diversifying the subset of the rSDRU residues selected in step c. with the set of amino acids selected in step d.;

    f. expressing the library of antibody VH or VL variants in a host or translating the library of antibody VH or VL variants in vitro; and

    g. selecting from the library of antibody VH or VL variants one or more affinity matured antibody having an improved affinity to an antigen.

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