Methods of Affinity Maturing Antibodies
First Claim
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1. A method of affinity maturing an antibody, comprising the steps of:
- a. obtaining an amino acid sequence of the antibody light chain variable region (VL) or the antibody heavy chain variable region (VH);
b. identifying rSDRU residues in the amino acid sequence of the antibody VL or VH;
c. selecting a subset of the rSDRU residues to be diversified;
d. selecting a set of amino acids or a universal set of amino acids used for diversifying the subset of the rSDRU residues;
e. preparing a library of antibody VL or VH variants by diversifying the subset of the rSDRU residues selected in step c. with the set of amino acids selected in step d.;
f. expressing the library of antibody VH or VL variants in a host or translating the library of antibody VH or VL variants in vitro; and
g. selecting from the library of antibody VH or VL variants one or more affinity matured antibody having an improved affinity to an antigen.
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Abstract
The invention relates to methods of affinity maturing antibodies.
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10 Claims
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1. A method of affinity maturing an antibody, comprising the steps of:
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a. obtaining an amino acid sequence of the antibody light chain variable region (VL) or the antibody heavy chain variable region (VH); b. identifying rSDRU residues in the amino acid sequence of the antibody VL or VH; c. selecting a subset of the rSDRU residues to be diversified; d. selecting a set of amino acids or a universal set of amino acids used for diversifying the subset of the rSDRU residues; e. preparing a library of antibody VL or VH variants by diversifying the subset of the rSDRU residues selected in step c. with the set of amino acids selected in step d.; f. expressing the library of antibody VH or VL variants in a host or translating the library of antibody VH or VL variants in vitro; and g. selecting from the library of antibody VH or VL variants one or more affinity matured antibody having an improved affinity to an antigen. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification