SYSTEM FOR DROPLET-BASED ASSAYS USING AN ARRAY OF EMULSIONS
First Claim
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1. A method of performing a droplet-based assay, comprising:
- obtaining an emulsion including droplets packed closely together in a three-dimensional packing arrangement;
reacting the emulsion with the droplets packed closely together; and
collecting data related to an analyte from individual droplets of the reacted emulsion as such droplets travel serially through a detection region.
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Abstract
System, including method and apparatus, for performing droplet-based assays. In the method, an emulsion may be obtained that includes droplets packed closely together in a three-dimensional packing arrangement. Data related to an analyte may be collected from individual droplets of the reacted emulsion as such droplets travel serially through a detection region.
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Citations
68 Claims
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1. A method of performing a droplet-based assay, comprising:
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obtaining an emulsion including droplets packed closely together in a three-dimensional packing arrangement; reacting the emulsion with the droplets packed closely together; and collecting data related to an analyte from individual droplets of the reacted emulsion as such droplets travel serially through a detection region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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29. A method of performing a droplet-based assay, comprising:
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producing a plurality of emulsions; thermocycling the emulsions in parallel while such emulsions are held in an array, to amplify one or more nucleic acid targets in droplets of the emulsions; driving flow of droplets of each emulsion through a detection region; and collecting data related to amplification of the nucleic acid targets from individual droplets of each emulsion as such droplets travel through the detection region. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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41. A method of performing a droplet-based assay, comprising:
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producing a plurality of emulsions; thermocycling the emulsions to amplify one or more nucleic acid targets in droplets of the emulsions; picking up droplets from the emulsions disposed in an array using an intake conduit disposed serially in each of the emulsions; driving flow of each emulsion through a detection region connected to the intake conduit; and collecting data related to amplification of the nucleic acid targets from individual droplets of each emulsion traveling through the detection region. - View Dependent Claims (42, 43)
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44. A system for performing a droplet-based assay, comprising:
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a plate including a plurality of emulsion production units capable of producing a plurality of emulsions in parallel; a thermocycler configured to thermally cycle the emulsions held in an array, to amplify one or more nucleic acid targets in the emulsions; a droplet transporter configured to pick up droplets of each emulsion from the array and to drive flow of the droplets through a detection region; and a detector configured to collect data related to amplification of the nucleic acid targets from individual droplets of the emulsions traveling through the detection region. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53)
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54. A system for performing a droplet-based assay, comprising:
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a plate including a plurality of emulsion production units capable of producing a plurality of emulsions in parallel; a droplet transporter configured to pick up droplets from the emulsions held in an array and to drive flow of the droplets through a detection region; a detector configured to collect data related to amplification of one or more nucleic acid targets from individual droplets of emulsions as such individual droplets travel through the detection region; and a controller programmed to determine, based on the data collected, whether each nucleic acid target is present or absent in individual droplets of the emulsions. - View Dependent Claims (55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65)
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66. A system for performing a droplet-based assay, comprising:
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a plate including a plurality of emulsion production units; an instrument configured to be operatively connected to the plate to drive production of emulsions collected in wells of the plate; a droplet transporter configured to pick up droplets from the emulsions held in an array and to drive flow of the droplets through a detection region; a detector configured to collect data related to one or more analytes from individual droplets of the emulsions as such individual droplets travel through the detection region; and a controller programmed to determine, based on the data collected, an aspect of the one or more analytes in one or more samples included in droplets of the emulsions. - View Dependent Claims (67, 68)
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Specification