Apparatus and Method for Optically Sensing Analyte Concentration in an Erythrocyte-Containing Fluid
First Claim
1. A sensor for determination of an analyte, comprising:
- a body of optical material;
an analyte-reactive reagent for the analyte, disposed on the optical material body in optical communication therewith; and
a plurality of particulate bodies dispersed through the analyte-reactive reagent, the particulate bodies being adapted to contribute to reflectance of light from the optical material body back into the optical material body through at least a portion of the analyte-reactive reagent, when the analyte-reactive reagent is in reaction with the analyte.
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Accused Products
Abstract
A device for determination of analytes has optical material with a deposit on a surface thereof. The deposit includes an analyte-reactive reagent and a particulate material for enhancing reflectance of a light beam emitted through the optical material into the analyte-reactive reagent. The enhanced reflectance provides a return of light through the optical material at a level which is not only analytically useful, but involves sufficient returned light intensity so as to enable markedly improved sensitivity and accuracy in analytical determinations. A suitable particulate material is one of hollow spherical particles. Moreover, the particulate material may be selected for a property such as a neutralizing charge that suppresses the membrane-disruptive effects of certain types of optical materials and hemolytic reagents on blood cells such as erythrocytes.
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Citations
44 Claims
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1. A sensor for determination of an analyte, comprising:
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a body of optical material; an analyte-reactive reagent for the analyte, disposed on the optical material body in optical communication therewith; and a plurality of particulate bodies dispersed through the analyte-reactive reagent, the particulate bodies being adapted to contribute to reflectance of light from the optical material body back into the optical material body through at least a portion of the analyte-reactive reagent, when the analyte-reactive reagent is in reaction with the analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A sensor for determination of at least one analyte in a fluid comprising:
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an optic fiber having proximal and distal ends, and further having a textured surface on a distal end; a coating comprising at least one analyte-reactive reagent disposed on the textured surface, said reagent producing an optically detectable change upon reaction with at least one analyte in a fluid to be presented to the distal end; and reflectance enhancement means for enhancing reflectance into the fiber from a sampling zone associated with said coating. - View Dependent Claims (19, 20, 21, 22)
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23. A device for measurement of an analyte in a fluid comprising:
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an optical material having a deposit on a surface thereof; wherein the deposit comprises an analyte-reactive reagent and a plurality of hollow polymeric particles; and wherein a sampling zone is associated with the'"'"'analyte-reactive reagent, a portion of the reagent being within the sampling zone and disposed between the surface of the optical material and the hollow polymeric particles, and the hollow polymeric particles providing for reflectance of a light beam emitted from the optical material into the sampling zone. - View Dependent Claims (24, 25, 26)
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27. A method of analyzing a fluid sample for an analyte comprising:
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presenting a fluid sample containing the analyte to a site on an optical material, the site having a deposit comprising an analyte-reactive reagent and a particulate means for enhancing reflectance; developing an optically detectable change in a reagent sampling zone associated with the analyte-reactive reagent; emitting a beam of light through the optical material into the reagent sampling zone; returning reflectance from the reagent sampling zone through the optical material to a detector; and measuring the reflectance for spectral qualities correlative to the analyte. - View Dependent Claims (28, 29, 30)
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31. A sensor for measuring concentration of an analyte in an erythrocyte-containing biological fluid comprising:
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an optical material having a region of analyte-reactive reagent for contact with a biological fluid to be analyzed; and a deposit comprising a plurality of negatively-charged particles disposed in the region; wherein said particles suppressing hemolysis of erythrocytes coming into contact with the region. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40)
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41. A method of manufacturing a sensor for determining an analyte, comprising:
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co-depositing an analyte-reactive reagent for the analyte and a plurality of particulate bodies into a sampling zone on an optical material body; and maintaining the analyte in optical communication with the optical material body. - View Dependent Claims (42, 43, 44)
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Specification