DETECTION DEVICES AND METHODS
First Claim
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1. A device for detecting the presence or absence of an analyte, the device comprising:
- a body including a flow path, a flow-through membrane, and a colorimetric sensor component disposed in or on the flow-through membrane;
wherein the colorimetric sensor comprises a polymerized composition comprising a diacetylene-containing polymer and a receptor, wherein the receptor is incorporated in the polymerized composition to form a transducer that provides a color change upon binding with one or more probes and/or analytes.
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Abstract
The application discloses embodiments of detection devices including a sensor component in a flow path between a first flow path portion and a second flow path portion. In embodiments described, the sensor component includes a receptor in a polymerized composition. The receptor is configured to bind with an analyte in a test sample. Upon binding the sensor component undergoes a detectable change in response to interaction of the analyte with the receptor.
59 Citations
52 Claims
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1. A device for detecting the presence or absence of an analyte, the device comprising:
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a body including a flow path, a flow-through membrane, and a colorimetric sensor component disposed in or on the flow-through membrane; wherein the colorimetric sensor comprises a polymerized composition comprising a diacetylene-containing polymer and a receptor, wherein the receptor is incorporated in the polymerized composition to form a transducer that provides a color change upon binding with one or more probes and/or analytes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 48, 49)
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13. A device for detecting the presence or absence of an analyte, the device comprising:
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a body including a flow path and a plurality of layers forming a multiple-layered structure, the flow path defined by a first flow passage portion and a second flow passage portion formed between a first layer and a second layer; and a sensor component disposed between the first and second layers, wherein the sensor component separates the first flow passage portion from the second flow passage portion. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A device for detecting the presence or absence of an analyte, the device comprising:
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a body including a flow path and a plurality of layers forming a multiple-layered structure, the flow path defined by a first flow passage portion and a second flow passage portion formed between a first layer and a second layer; a patterned layer interposed between the first layer and the second layer, wherein the patterned layer forms a chamber, the first flow passage portion, and the second flow passage portion, and a sensor component disposed in the chamber formed by the patterned layer. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A device comprising:
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a sample flow path; a zone including a sensor component; one or more reagents for sample preparation disposed in one or more distinct zones of the sample flow path ahead of the sensor component; and optionally, a probe disposed in a distinct zone of the sample flow path ahead of the sensor component and different from the one or more sample preparation reagents. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 50)
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47. A device for sample preparation and analysis of a target analyte, the device comprising:
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a sample flow path; one or more reagents for sample preparation disposed in one or more distinct zones of the sample flow path; a zone including a probe disposed in the sample flow path downstream from at least one of the sample preparation reagents; and a zone including a colorimetric sensor component, wherein the colorimetric sensor comprises a polymerized composition comprising a diacetylene-containing polymer and a receptor, wherein the receptor is incorporated in the polymerized composition to form a transducer that provides a color change upon binding with one or more probes and/or analytes.
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51. A method of detecting the presence or absence of an analyte, the method comprising:
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providing a device comprising; a body including a flow path and a plurality of layers forming a multiple-layered structure, the flow path defined by a first flow passage portion and a second flow passage portion formed between a first layer and a second layer; and a flow-through membrane disposed between the first and second layers, wherein the flow-through membrane separates the first flow passage portion from the second flow passage portion; providing a test sample suspected of containing one or more target analytes; optionally, providing one or more probes suitable for an indirect assay of the one or more target analytes; providing a sensor component; combining the test sample, optional probes, and sensor component to form a mixture; inducing flow of the mixture from the first flow passage portion to the second flow passage portion across the flow-through membrane to collect the sensor component and bound target analytes and/or probes; and discerning the detectable change in the sensor component upon binding with the target analytes and/or probes.
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52. A method of detecting the presence or absence of an analyte, the method comprising:
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providing a device comprising; a body including a flow path and a plurality of layers forming a multiple-layered structure, the flow path defined by a first flow passage portion and a second flow passage portion formed between a first layer and a second layer; a patterned layer interposed between the first layer and the second layer, wherein the patterned layer forms a chamber, the first flow passage portion, and the second flow passage portion, and a flow-through membrane disposed in the chamber formed by the patterned layer; providing a test sample suspected of containing one or more target analytes; optionally, providing one or more probes suitable for an indirect assay of the one or more target analytes; providing a sensor component; combining the test sample, optional probes, and sensor component to form a mixture; inducing flow of the mixture from the first flow passage portion to the second flow passage portion across the flow-through membrane in the chamber to collect the sensor component and bound target analytes and/or probes; and discerning the detectable change in the sensor component upon binding with the target analytes and/or probes.
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Specification