TRIPLE FUNCTION ELECTRODES

• US 20110100820A1
• Filed: 03/20/2009
• Published: 05/05/2011
• Est. Priority Date: 05/23/2008
• Status: Abandoned Application

• Current Assignee: ITI Scotland Limited (Scottish Government)
• Original Assignee: ITI Scotland Limited (Scottish Government)
• Inventors: Walton, Anthony, Bachmann, Till, Mount, Andrew
• Application Number:
  US12/994,424
• Publication Number:
  US 20110100820A1
• Time in Patent Office: Days
• US Class Current: 204/547
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2400/0424 Dielectrophoretic forces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]

Abstract

Provided is a device for assaying one or more analytes, said device comprising an electrode, a means for optical detection; and a means for electrochemical detection, wherein the device is configured such that the electrode is capable of promoting transport of an analyte when a field is applied to the analyte via the electrode, and wherein the means for electrochemical detection employs the electrode and the means for optical detection employs the electrode, and wherein the device is configured to carry out dielectrophoresis.

Further provided is the use of the device of the present invention for promoting transport of an analyte, detecting the optical properties of the analyte and detecting the electrochemical properties of the analyte.

Also provided is method for assaying one or more analytes, which method comprises the steps of: promoting transport of an analyte, performing an optical measurement of the analyte and performing an electrochemical measurement of the analyte, which method employs the device of the present invention.

Claims

1. A device for assaying one or more analytes, said device comprising:

(a) a plurality of electrodes;

(b) a means for optical detection; and

(c) a means for electrochemical detection;

wherein the device is configured such that the electrode is capable of promoting transport of an analyte when a field is applied to the analyte via the electrode; and

wherein the means for electrochemical detection employs the electrode; and

the means for optical detection employs the electrode and wherein the device is configured to carry out dielectrophoresis; and

wherein the plurality of electrodes is in the form of an interdigitated electrode structure.

2. The device of claim 1, wherein the electrode is composed of an optically transparent material.

3. The device of claim 2, wherein the optically transparent material is ITO.

4. A device according to claim 1, wherein the device is suitable for attaching an analyte to the electrode, detecting the optical properties of the analyte and detecting the electrochemical properties of the analyte.

5. A device according to claim 4 wherein the optical detection and electrochemical detection can either be simultaneous or sequential.

6. A device according to claim 1, wherein the electrode further comprises a capture probe, which capture probe is capable of reacting with the analyte to capture the analyte on the electrode.

7. A device according to claim 1, wherein the device is suitable for detecting the presence or absence of an analyte in a sample, purifying the analyte in the sample, isolating the analyte in the sample or sorting the analyte in the sample.

8. A device according to claim 1, wherein the device is suitable for detecting the presence of the analyte in a sample and optionally quantifying the analyte.

9. A device according to claim 1, wherein the device is configured to apply at least two alternating fields, wherein at least one alternating field is composed of a plurality of pulses to influence a sample and/or the electrode or capture probe capable of binding an analyte.

10. A device according to claim 9, wherein the at least two alternating fields are applied simultaneously or sequentially.

11. A device according to claim 9, wherein each alternating field has a combination of frequency, pulse duration and pulse rise time that is unique in relation to that combination for all other alternating fields.

12. A device according to claim 9, wherein the first alternating field has a frequency of 1 to 109 Hz.

13. A device according to claim 9, wherein the first alternating field has a field strength of 10 kV/m to 100 MV/m.

14. A device according to claim 9, wherein the second alternating field is capable of promoting binding of the analyte to the binding phase.

15. A device according to claim 9, wherein the second alternating field has a pulse duration of 10⁻

2 s to 10<sup>−

8</sup> s.

16. A device according to claim 9, wherein the second alternating field has a pulse rise time of 10⁻

8 s to 10<sup>−

19</sup> s.

17. A device according to claim 9, wherein the second alternating field has a frequency of 10² to 10⁹ Hz.

18. A device according to claim 9, wherein the second alternating field has a voltage of 10 mV to 5 V.

19. A device according to claim 9, wherein the first alternating field and second alternating field have waveforms independently selected from sinusoidal, square, sawtooth and triangular.

20. A device according to claim 1, wherein the analyte comprises one or more compounds selected from a cell, a protein, a polypeptide, a peptide, a peptide fragment, an amino acid, polynucleotides such as DNA or RNA, oligonucleotides, nucleotides, natural and synthetic chemicals and metabolites.

21. A device according claim 1 wherein the analyte is labelled with one or more labels relatable to the analyte which are suitable for optical detection.

22. A device according to claim 21, wherein the labels are selected from nanoparticles, single molecules, chemiluminescent enzymes and fluorophores.

23. A device according to claim 22, wherein the labels are nanoparticles comprising a collection of molecules and/or atoms.

24. A device according to claim 22, wherein the nanoparticles are selected from metals, metal nanoshells, metal binary compounds and quantum dots.

25. A device according to claim 22, wherein the nanoparticles are metal compounds selected from CdSe, ZnS, CdTe, CdS, PbS, PbSe, HgI, ZnTe, GaAs, HgS, CdAs, CdP, ZnP, AgS, InP, GaP, GaInP, and InGaN.

26. A device according to claim 22, wherein the nanoparticles are selected from gold, silver, copper, cadmium, selenium, palladium and platinum.

27. A device according to claim 22, wherein the nanoparticles are less than 100 nm in diameter.

28. A device according to claim 27, wherein the nanoparticles are 5-50 nm in diameter.

29. A device according to claim 28, wherein the nanoparticles are 10-30 nm in diameter.

30. A device according to claim 21, wherein the one or more labels for each different analyte have different physical properties.

31. A device according to claim 30, wherein the physical properties are selected from one or more of size, shape and surface roughness.

32. A device according to claim 21, wherein the labels for each different analyte have different compositions.

33. A device according to claim 21, wherein the labels for each different analyte are of different types.

34. A device according to claim 1, wherein the means for optical detection is configured to carry out any of optical emission detection, optical absorbance detection, optical scattering detection, spectral shift detection, surface plasmon resonance imaging, total internal reflection fluorescence and surface-enhanced Raman scattering from adsorbed dyes.

35. A device according to claim 34 wherein when the means for optical detection is configured to carry out optical emission detection the device is further configured to irradiate the labelled analytes with light capable of exciting the labels and detecting the frequency and/or intensity of light emissions from the labels.

36. A device according to claim 35, wherein the light is laser light.

37. A device according to claim 35, wherein the light is selected from infra-red light, visible light and UV light.

38. A device according to claim 37, wherein the light is white light.

39. A device according to claim 1, wherein the means for electrochemical detection is configured to carry out electrochemical impedance spectroscopy.

44. A method for assaying one or more analytes, which method comprises the steps of:

a) promoting transport of an analyte

b) performing an optical measurement of the analyte

c) performing an electrochemical measurement of the analyte;

wherein said method employs the device of claim 1.

45. The method of claim 44 wherein the steps of performing an optical measurement of the analyte and performing an electrochemical measurement of the analyte are carried out either simultaneously or sequentially.

40-43. -43. (canceled)