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QUANTITATIVE NUCLEASE PROTECTION SEQUENCING (qNPS)

  • US 20110104693A1
  • Filed: 11/03/2010
  • Published: 05/05/2011
  • Est. Priority Date: 11/03/2009
  • Status: Abandoned Application
First Claim
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1. A method of detecting at least one target in a biological sample comprising(i) contacting said sample with at least one nuclease protection probe (NPP) which specifically binds to said target,(ii) exposing said sample to one or more reagents under conditions that are effective to eliminate any unbound NPP,(iii) optionally separating the bound NPP from the target, and(iv) sequencing said NPP, a complement thereof, or a molecule incorporating said NPP or a compliment.

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