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Analysis of Circulating Tumor Cells, Fragments, and Debris

  • US 20110104718A1
  • Filed: 11/02/2010
  • Published: 05/05/2011
  • Est. Priority Date: 08/23/2001
  • Status: Active Grant
First Claim
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1. A method for diagnosing disease in a test subject comprising:

  • a. obtaining a biological specimen from a test subject, said specimen comprising a mixed cell population suspected of containing intact rare cells and further comprising;

    i. cell fragments derived from rare cells, orii. cellular debris derived from rare cells;

    b. preparing a magnetically-labeled sample wherein said biological sample is mixed with magnetic particles coupled to a first biospecific ligand which reacts specifically with said intact rare cells, and said cell fragments or said cellular debris, to the substantial exclusion of other specimen components;

    c. contacting said magnetically-labeled sample with at least one additional biospecific ligand which specifically labels said intact rare cells, and said cell fragments or said cellular debris, to the substantial exclusion of other specimen components;

    d. analyzing said labeled rare cells, and said labeled cell fragments or said labeled cellular debris, the presence of said labeled rare cells, said labeled cell fragments, and said labeled cellular debris indicating the presence of disease.

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