BLOOD VISCOSITY ANALYSIS

• US 20110104738A1
• Filed: 03/31/2009
• Published: 05/05/2011
• Est. Priority Date: 04/01/2008
• Status: Active Grant

• Current Assignee: Tommy Forsell
• Original Assignee: Tommy Forsell
• Inventors: Forsell, Tommy
• Granted Patent: US 8,460,938 B2
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: G01N 11/04 through a restricted passage, e.g. tube, aperture
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/06 Investigating concentration of particle suspensions by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10
• CPC Class Codes: G01N 15/075 by optical means
• CPC Class Codes: G01N 2015/0003 Determining electric mobility, velocity profile, average speed or velocity of a plurality of particles
• CPC Class Codes: G01N 2015/0065 biological, e.g. blood
• CPC Class Codes: G01N 2015/0092 Monitoring flocculation or agglomeration
• CPC Class Codes: G01N 2015/0693 by optical means, e.g. by integrated nephelometry
• CPC Class Codes: G01N 2021/1727 Magnetomodulation
• CPC Class Codes: G01N 21/1717 with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
• CPC Class Codes: G01N 21/51 inside a container, e.g. in an ampoule G01N21/53 takes precedence
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/54

Abstract

A blood analyzing device (100) comprises a holder (110) for receiving a cuvette (20) with a blood sample (30). The device (100) induces a movement of the blood cells in the sample (30) by applying a field (230) over the cuvette (20). A detector (130) detects, based on output light from the blood sample (30) originating from a light source (120), a change in the induced movement of the cells caused by an increase in viscosity of the blood due to an aggregation and/or coagulation process. A processor (140) processes the detector reading and generates a signal representative of the hemostasis function of the tested blood.

Claims

1. A blood analyzing device comprising:

a holder arranged for carrying a container having a cuvette comprising a blood sample;

a unit for inducing movement of blood cells in said blood sample when said container is positioned in said holder;

a light source arranged for providing input light of a wavelength at which a hemoglobin species has maximum absorbance into said blood sample;

a detector arranged for i) detecting, at least once within a measuring time interval following positioning of said container in said holder, output light having passed through said blood sample, ii) detecting. based on said output light from said blood sample and following said measuring time interval, a change in said induced movement of said blood cells in said blood sample as a reduction in a rate of change of absorbance at said wavelength at which said hemoglobin species has maximum absorbance and iii) generating a detection signal based on said detected change in said induced movement; and

a processor connected to said detector and arranged for i) determining at least one of total hemoglobin or at least one hemoglobin fraction in said blood sample based on said output fight detected during said measuring time interval and ii) determining a viscosity representing signal representative of a viscosity change in said blood sample based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction.

2. The device according to claim 1, wherein said unit for inducing movement comprises a field applier arranged for applying a field of varying direction across said blood sample to induce movement of said blood cells in said blood sample.

3. The device according to claim 2, wherein said field applier is arranged for applying an electrical field of varying direction across said blood sample to induce movement of said blood cells in said sample.

4. The device according to claim 2, wherein said field applier is arranged for applying a magnetic field of varying direction across said blood sample to induce movement of said blood cells in said blood sample.

5. The device according to claim 4, wherein said field applier comprises:

a set of multiple electromagnets positioned at different positions around said blood sample when said container is positioned in said holder; and

a field activator arranged for selectively activating said multiple electromagnets to provide said magnetic field of varying direction across said blood sample.

6. The device according to claim 5, wherein said field activator is arranged for selectively switching on and off said multiple electromagnets in a clockwise or counter-clockwise manner as positioned around said blood sample.

7. The device according to claim 4, wherein said cuvette comprises said blood sample and ferromagnetic objects suspended in said blood sample and said field applier comprises:

a set of multiple electromagnets positioned at different positions around said blood sample when said container is positioned in said holder; and

a field activator arranged for selectively activating said multiple electromagnets and selectively switching direction of a first magnetic field applied by a first electromagnet of a pair and selectively switching direction of a second magnetic field applied by a second electromagnet of said pair, said first electromagnet and said second electromagnetic are positioned on either sides of said blood sample and said direction of said first magnetic field being opposite to said direction of said second magnetic field.

8. The device according to claim 7, wherein said field activator is arranged for increasing a field strength of said first magnetic field and second magnetic field, said detector is arranged for detecting a stretching-pressing movement of a blood clot in said blood sample induced by said pair of electromagnets, and said processor is arranged for generating a clot strength signal based on a field strength of at least one of said first magnetic field and said second magnetic field when said detector detects said stretching-pressing movement and based on said at least one of total hemoglobin or at least one hemoglobin fraction.

9. The device awarding to claim 2, wherein said field applier is arranged for initially applying said field at a first field strength and for applying said field at a second field strength following a detection of a viscosity change in said blood sample caused by aggregation of thrombocytes in said blood sample, said second field strength being larger said first field Strength.

10. The device according to claim 9, wherein said processor is arranged for generating an aggregation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction, said field applier is responsive to said aggregation detection signal and switches from said first field strength to said second field strength upon reception of said aggregation detection signal.

11. The device according to claim 9, wherein said processor is arranged for generating a coagulation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction, said predefined viscosity change being detectable following said field applier switching to said second field strength.

12. The device according to claim 2, wherein said field applier is arranged for applying pressure waves of varying direction into said blood sample to induce movement of said blood cells in said blood sample.

13. The device according to claim 12, wherein said field applier is arranged for initially applying said pressure waves at with a first amplitude and at a first frequency and for applying said pressure waves at a second amplitude and/or at a second frequency following a detection of a viscosity change in said blood sample caused by aggregation of thrombocytes in said blood sample.

14. The device according to claim 13, wherein said processor is arranged for generating an aggregation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction, said field applier is responsive to said aggregation detection signal and switches from said first amplitude and said first frequency to said second amplitude and/or said second frequency upon reception of said aggregation detection signal.

15. The device according to claim 13, wherein said processor is arranged for generating a coagulation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction, said predefined viscosity change being detectable following said field applier switching to said second amplitude and/or said second frequency.

16. The device according to claim 12, wherein said field applier comprises:

a set of multiple ultrasound transducers positioned at different positions around said blood sample when said container is positioned in said holder; and

a field activator arranged for selectively activating said multiple ultrasound transducers to provide ultrasound pressure waves of varying direction across said blood sample.

17. The device according to claim 16, wherein said field activator is arranged for selectively switching on and off said multiple ultrasound transducers in a clockwise or counter-clockwise manner as positioned around said blood sample.

18. A method of analyzing a blood sample contained in a cuvette of a container, said method comprising:

arranging said container in a holder of a blood analyzing device;

providing input light from a light source of a wavelength at which a hemoglobin species has maximum absorbance into said blood sample;

detecting, at least once within a measuring time interval following positioning of said container in said holder, output light having passed through said blood sample;

determining at least one of total hemoglobin or at least one hemoglobin fraction in said blood sample based on said output light detected during said measuring time interval;

inducing movement of blood cells in said blood sample;

detecting, based on said output light from said blood sample and following said measuring time interval, a change in said induced movement of said blood cells in said blood sample as a reduction in a rate of change of absorbance at said wavelength at which said hemoglobin species has maximum absorbance;

generating a detection signal based on said detected change in said induced movement;

and

determining a viscosity representing signal representative of a viscosity change in said blood sample based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction.

19. The method according to claim 18, wherein inducing said movement comprises providing a field of varying direction across said blood sample to induce movement of said blood cells in said blood sample.

20. The method according to claim 19, wherein providing said field comprises providing a magnetic field of varying direction across said blood sample to induce movement of said blood cells in said blood sample.

21. The method according to claim 20, further comprising:

suspending ferromagnetic objects in said blood sample;

, selectively switching direction of a first magnetic field applied by a first electromagnet of a pair; and

selectively switching direction of a second magnetic field applied by a second electromagnet of said pair, said first electromagnet and said second electromagnetic are positioned on either sides of said blood sample and said direction of said first magnetic field being opposite to said direction of said second magnetic field;

22. The method according to claim 21, further comprising:

increasing a field strength of said first magnetic field and second magnetic field;

detecting a stretching-pressing movement of a blood clot in said blood sample induced by said pair of electromagnets; and

generating a clot strength signal based on a field strength of at least one of said first magnetic field and said second magnetic field when said stretching-pressing movement is being detected.

23. The method according to claim 19, wherein providing said field comprises:

initially providing said field at a first field strength;

providing said field at a second field strength following a detection of a viscosity change in said blood sample caused by aggregation of thrombocytes in said blood sample, said second field strength being larger said first field strength, wherein determining said viscosity representing signal comprises generating an aggregation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal, and providing said field at said second field strength is performed in response to said aggregation detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction.

24. The method according to claim 19, wherein providing said field comprises:

initially providing said field at a first field strength;

providing said field at a second field strength following a detection of a viscosity change in said blood sample caused by aggregation of thrombocytes in said blood sample, said second field strength being larger said first field strength, wherein

determining said viscosity representing signal comprises generating a coagulation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction, said predefined viscosity change being detectable following application of said second field strength.

25. The method according to claim 19, wherein providing said field comprises applying pressure waves of varying direction into said blood sample to induce movement of said blood cells in said blood sample.

26. The method according to claim 25, wherein applying said pressure waves comprises:

initially applying said pressure waves at with a first amplitude and at a first frequency;

and

applying pressure waves at a second amplitude and/or at a second frequency following a detection of a viscosity change in said blood sample caused by aggregation of thrombocytes in said blood sample, wherein determining said viscosity representing signal comprises generating an aggregation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction, and applying said pressure waves at said second amplitude and/or said second frequency is performed in response to said aggregation detection signal.

27. The method according to claim 25, wherein applying said pressure waves comprises:

initially applying said pressure waves at with a first amplitude and at a first frequency;

and

applying pressure waves at a second amplitude and/or at a second frequency following a detection of a viscosity change in said blood sample caused by aggregation of thrombocytes in said blood sample, wherein determining said viscosity representing signal comprises generating a coagulation detection signal based on detection of a predefined change in viscosity of said blood sample as determined based on said detection signal and said at least one of total hemoglobin or at least one hemoglobin fraction, said predefined viscosity change being detectable following applying said pressure waves at said second amplitude and/or said second frequency.

28-39. -39. (canceled)