Methods and Apparatus for Conducting Multiple Measurements on a Sample
First Claim
1. An assay module for conducting luminescence assays comprising a plurality of assay domains, said assay domains comprising a first assay domain having a first reagent and a second assay domain having a second reagent, wherein said first assay domain is capable of generating luminescence at least 10 times brighter than said second assay domain and said first assay domain and said second assay domains are not adjacent and/or are separated by at least one other assay domain to reduce luminescence emitted from said first assay domain from interfering with luminescence emitted from said second assay domain.
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Abstract
Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.
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Citations
23 Claims
- 1. An assay module for conducting luminescence assays comprising a plurality of assay domains, said assay domains comprising a first assay domain having a first reagent and a second assay domain having a second reagent, wherein said first assay domain is capable of generating luminescence at least 10 times brighter than said second assay domain and said first assay domain and said second assay domains are not adjacent and/or are separated by at least one other assay domain to reduce luminescence emitted from said first assay domain from interfering with luminescence emitted from said second assay domain.
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10. An assay module comprising a plurality of assay domains for measuring a panel of analytes, said panel comprising a plurality of analytes that are members of an analyte category selected from cytokines and/or their receptors;
- growth factors and/or their receptors;
second messengers;
drugs of abuse;
therapeutic drugs;
auto-antibodies;
allergen specific antibodies;
tumor markers;
cardiac markers;
markers associated with hemostasis;
markers of acute viral hepatitis infection;
markers of Alzheimers Disease;
markers of osteoporosis;
markers of fertility;
markers of congestive heart failure;
markers of thyroid disorders;
markers of prostrate cancer;
nucleic acid arrays for measuring mRNA levels of mRNA coding for cytokines, growth factors, components of the apoptosis pathway, expression of the P450 enzymes or expression of tumor related genes;
nucleic acid arrays for genotyping individuals, pathogens, or tumor cells. - View Dependent Claims (11, 12)
- growth factors and/or their receptors;
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13. A method for detecting or correcting for a source of assay error in an assay for an analyte comprising:
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(a) introducing a sample comprising analyte into an assay module comprising (i) a first assay domain comprising a first reagent capable of binding said analyte; and (ii) a second assay domain comprising said first reagent; wherein said first assay domain and said second binding domain having different properties, said different properties selected from the group consisting of surface area, reagent concentration, heat stability, pH stability, hydrophobic or hydrophilic properties, co-reactants, epitope specificity, binding kinetics, non-specific binding properties, binding constants, and/or thermodynamics; and (b) comparing the binding of the analyte to the first and second assay domains so as to detect or correct for sources of assay error.
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14. An assay module for conducting an assay on a sample comprising an analyte and at least one interferent, said module comprising:
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(a) an assay domain capable of binding said analyte; and (b) a sequestering domain capable of binding said interferent. - View Dependent Claims (15, 16)
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- 17. An assay module comprising a plurality of assay domains, wherein said plurality of assay domains comprises one or more assay domains comprising one or more binding reagents and further comprising a first control domain comprising pre-bound label, a second control domain providing a defined reaction and a third control domain consisting essentially of blocking agent.
- 21. An assay module comprising one or more assay domains comprising assay reagents and one or more sequestrating domains comprising sequestration agents for sequestering interfering species.
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23. An assay module comprising one or more assay domains comprising assay reagents and one or more sequestrating domains comprising sequestration agents capable of sequestering a labeled reagent.
Specification