COMPOSITIONS AND METHODS FOR PROVIDING SUBSTANCES TO AND FROM AN ARRAY

• US 20110105356A1
• Filed: 05/05/2009
• Published: 05/05/2011
• Est. Priority Date: 05/07/2008
• Status: Abandoned Application

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Yang, Robert, DeRosier, Chad F., Ren, Hongji, Black, Fiona E., Heiner, David L., Moon, John A.
• Application Number:
  US12/991,121
• Publication Number:
  US 20110105356A1
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C40B 50/14 Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

Methods, kits, systems, and multilayer transfer media for transferring a substance to and from an array are disclosed herein. Also disclosed herein are methods of detecting a substance that has been transferred to an array.

Claims

1-105. -105. (canceled)

106. A method of transferring a molecule to an array, said method comprising:

obtaining an array having a surface, wherein said array comprises a plurality of capture probes;

obtaining a preformed porous material, wherein said preformed porous material comprises said molecule; and

providing said preformed porous material to said surface such that said material is in fluid contact with said surface, thereby transferring said molecule to said array.

107. The method of claim 106, wherein said preformed porous material comprises a fibrous material.

108. The method of claim 106, wherein said preformed porous material comprises a gel matrix.

109. The method of claim 106, wherein said preformed porous material is attached to a non-porous backing.

110. The method of claim 106, wherein said molecule is dissolved or suspended in a liquid.

111. The method of claim 106, wherein said molecule is dried or lyophilized.

112. The method of claim 106, wherein said molecule is a protein.

113. The method of claim 112, wherein said protein is an enzyme used for nucleic acid sequencing.

114. The method of claim 106, wherein said providing the preformed porous material to the surface further comprises applying pressure to the porous material.

115. The method of claim 106, further comprising performing a binding reaction by allowing the molecule to bind with at least one of said capture probes.

116. The method of claim 115, wherein said preformed porous material is in fluid contact with the surface during the binding reaction.

117. The method of claim 115, wherein said preformed porous material comprises different molecules that bind to different capture probes of said plurality of capture probes.

118. The method of claim 106, further comprising removing said preformed porous material from said array, thereby removing fluid from said array.

119. The method of claim 106, further comprising providing an additional porous material to said array.

120. The method of claim 119, wherein said additional porous material contacts said preformed porous material.

121. The method of claim 119, wherein said preformed porous material is removed prior to providing said additional porous material to said array.

122. The method of claim 106, wherein said preformed porous material is smaller than the area of said surface of the array.

123. A kit for transferring a molecule to an array, said kit comprising:

an array having a surface, wherein said surface comprising a plurality of capture probes; and

a preformed porous material, wherein said preformed porous material comprises a molecule.

124. A method for detecting a molecule, said method comprising:

obtaining an array having a surface, wherein said array comprises a plurality of capture probes;

obtaining a preformed porous material, wherein said preformed porous material comprises said molecule;

providing said preformed porous material to said surface such that said preformed porous material is in fluid contact with said surface, whereby said molecule becomes bound to at least one of said capture probes of said array; and

detecting said molecule bound to said capture probe.

125. A array system comprising:

an array having a surface, wherein said array comprises a plurality of capture probes; and

a preformed porous material comprising a molecule, said preformed porous material being in fluid contact with said array.