YEAST CELLS AND METHODS FOR INCREASING ETHANOL PRODUCTION
First Claim
1. A method for producing ethanol comprising:
- fermenting a composition with a yeast, wherein the composition comprises pretreated solid lignocellulosic material, wherein the pretreated solid lignocellulosic material is present at a concentration of at least 12% solids, and wherein at least 30 grams ethanol per liter are produced.
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Abstract
Provided herein are methods for producing ethanol using yeast to ferment pretreated solid lignocellulosic materials such as pretreated solid lignocellulosic materials obtained from softwoods like pine. The pretreated solid lignocellulosic material is present at a concentration of at least 12% solids, and at least 30 grams ethanol per liter, preferably at least 40 grams ethanol per liter, are produced within 48 hours. In some aspects, the pretreated solid lignocellulosic material is not detoxified prior to the fermentation. Also provided herein are yeast that ferment a composition that includes pretreated solid lignocellulosic material at a concentration of at least 12% solids to yield at least 30 grams ethanol per liter, preferably at least 40 grams ethanol per liter, in 48 hours. Further provided herein are methods for acclimatizing yeast.
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Citations
35 Claims
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1. A method for producing ethanol comprising:
fermenting a composition with a yeast, wherein the composition comprises pretreated solid lignocellulosic material, wherein the pretreated solid lignocellulosic material is present at a concentration of at least 12% solids, and wherein at least 30 grams ethanol per liter are produced. - View Dependent Claims (2, 3, 4, 6, 9, 10, 11, 12, 14, 15, 16, 17)
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5. (canceled)
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7. (canceled)
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8. (canceled)
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13. (canceled)
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18. (canceled)
- 19. A yeast that ferments a composition comprising pretreated solid lignocellulosic material at a concentration of at least 12% solids to yield at least 30 grams ethanol per liter in 48 hours.
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22. (canceled)
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25. An isolated yeast AJP50 deposited with the American Type Culture Collection in accordance with the provisions of the Budapest Treaty.
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26. A method for acclimatizing yeast comprising:
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culturing yeast in a first composition comprising pretreated solid lignocellulosic material; transferring a sample comprising the cultured yeast to a second composition comprising pretreated solid lignocellulosic material and culturing the yeast in the second composition; repeating the transferring until the yeast will produce at least 30 grams ethanol per liter during a batch simultaneous saccharification and fermentation with at least 12% solids. - View Dependent Claims (27, 28, 29, 30, 31, 33, 34, 35)
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32. (canceled)
Specification