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PROBE FOR NUCLEIC ACID SEQUENCING AND METHODS OF USE

  • US 20110111975A1
  • Filed: 12/29/2010
  • Published: 05/12/2011
  • Est. Priority Date: 12/12/2005
  • Status: Abandoned Application
First Claim
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1. A probe for sequencing a nucleic acid molecule, comprising:

  • a polymerizing agent having an active site capable of binding to a target nucleic acid molecule and promoting synthesis of a complementary nucleic acid molecule that elongates as complementary nucleotides are incorporated into the complementary nucleic acid molecule; and

    one or more molecular linkers spaced apart on the polymerizing agent,wherein the molecular linkers are attached to the polymerizing agent via a tether;

    wherein the molecular linkers form a symmetrical branch structure, andwherein each branch carries a different nucleotide analog that is capable of reversibly binding to the target nucleic acid molecule, without being detached from the linker, by specifically binding with a complementary nucleotide in the target nucleic acid molecule, wherein specific binding of the nucleotide analog on the linker with a complementary nucleotide in the target nucleic acid molecule is indicated by emission of a characteristic signal that indicates pairing of the nucleotide analog on the linker with its complementary nucleotide.

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