CELL LINE, SYSTEM AND METHOD FOR OPTICAL CONTROL OF SECONDARY MESSENGERS
First Claim
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1. A method for generating secondary messengers in a cell, the method comprising:
- modifying a nucleotide sequence for expressing a chimeric light responsive rhodopsin-based membrane protein with one or more heterologous receptor subunits; and
expressing the light responsive membrane protein in a cell for producing a secondary messenger in response to light.
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Abstract
A variety of methods, devices and compositions are implemented for light-activated molecules. One such method is implemented for generating secondary messengers in a cell. A nucleotide sequence for expressing a chimeric light responsive membrane protein (e.g., rhodopsin) is modified with one or more heterologous receptor subunits {e.g., an adrenergic receptor (alpha1, Beta2)}. The light responsive membrane protein is expressed in a cell for producing a secondary messenger in response t light.
162 Citations
12 Claims
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1. A method for generating secondary messengers in a cell, the method comprising:
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modifying a nucleotide sequence for expressing a chimeric light responsive rhodopsin-based membrane protein with one or more heterologous receptor subunits; and expressing the light responsive membrane protein in a cell for producing a secondary messenger in response to light. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for assessing the efficacy of a putative treatment regimen relating to intracellular messengers, the method comprising:
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modifying a nucleotide sequence for expressing a chimeric light responsive rhodopsin-based membrane protein with one or more heterologous receptor subunits; expressing the light responsive membrane protein in a cell for producing a secondary messenger in response to light; exposing the protein to light; and assessing the effects of the treatment.
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9. A cell expressing a chimeric light responsive rhodopsin-based membrane protein with one or more heterologous receptor subunits
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10. The cell of claim 9, wherein the one or more heterologous receptor subunits are adrenergic receptors of at least one of alpha1 and Beta2.
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10-1. A nucleotide sequence for expressing a chimeric light responsive membrane rhodopsin-based protein with one or more heterologous receptor subunits.
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11. A nucleotide sequence according to Seq. Id. No. 1 or Seq. Id. No. 3 and point mutations thereof.
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12. A cell expressing the amino acid according to Seq. Id. No. 2 or Seq. Id No. 4 and point mutations thereof.
Specification