Methods For Producing Enriched Populations of Human Retinal Pigment Epithelium Cells For Treatment of Retinal Degeneration
First Claim
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8. A method for the spontaneous differentiation of hES cells into cells RPE, RPE-like, or RPE progenitor cells, said method comprising:
- a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells;
b) culturing the multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; and
c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining human RPE cells.
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Abstract
This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
47 Citations
24 Claims
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8. A method for the spontaneous differentiation of hES cells into cells RPE, RPE-like, or RPE progenitor cells, said method comprising:
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a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells; b) culturing the multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; and c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining human RPE cells. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 10, 18, 19, 20, 21, 22, 23, 24)
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9. (canceled)
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11-17. -17. (canceled)
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