Highly Simplified Lateral Flow-Based Nucleic Acid Sample Preparation and Passive Fluid Flow Control
First Claim
1. A lateral flow sample preparation (LFSP) device comprising a lateral flow matrix which defines a flow path and which comprises in series the following elements:
- a sample receiving zone for receiving an aliquot of a fluid sample; and
,an immuno-capture zone in lateral flow contact with said sample receiving zone, which contains immobilized antibody reactive with a ligand present on a biological particle or cell of interest.
7 Assignments
0 Petitions
Accused Products
Abstract
Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.
68 Citations
30 Claims
-
1. A lateral flow sample preparation (LFSP) device comprising a lateral flow matrix which defines a flow path and which comprises in series the following elements:
-
a sample receiving zone for receiving an aliquot of a fluid sample; and
,an immuno-capture zone in lateral flow contact with said sample receiving zone, which contains immobilized antibody reactive with a ligand present on a biological particle or cell of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 21, 22)
-
- 16. A device comprising a lateral flow matrix which defines a flow path and which comprises a geometrically-defined absorbent material capable of supporting fluid wicking and the passive control of at least one fluid flow therein.
-
20. A geometrically-defined nitrocellulose strip having a configuration as shown in
FIG. 5 , 6, 7, 8, 9 or 14A.
-
24. A method for measuring an amount of a target, the method comprising the steps of:
-
disposing a plurality of biological particles comprising one or more targets in a sample receiving zone; lysing the particles; and binding the targets to a first ligand in a capture zone in lateral flow connection with the sample receiving zone, thereby increasing a concentration of the target relative to other constituents in the particles. - View Dependent Claims (25, 26, 27, 28, 29, 30)
-
Specification