DYNAMIC THERMORESPONSIVE NANOPARTICLES FOR STABILIZATION OF ENZYMES AT HIGH TEMPERATURES
First Claim
1. A nanocatalyst comprising one or more proteins, said proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell.
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Abstract
The present invention provides a thermoresponsive nanoparticle useful for the stabilization of enzymes in environments having a temperature greater than thirty degrees Centigrade. The thermoresponsive nanoparticle has (a) a functionalized enzyme conjugate having one or more enzymes or biological catalysts, the enzymes or biological catalysts are modified with palmitic acid N-hydroxysuccinimide ester and acryclic acid N-hydroxysuccinimide ester, and (b) a thermally responsive polymer, wherein the functionalized enzyme conjugate is encapsulated within the thermally responsive polymer. A nanocatalyst is provided that has one or more proteins. The proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell. The proteins are one or more enzymes or biological catalysts. A method for protecting the proteins is also set forth.
9 Citations
29 Claims
- 1. A nanocatalyst comprising one or more proteins, said proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell.
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14. A method for protecting proteins from environmental conditions comprising:
- immobilizing one or more proteins and encapsulating said one or more immobilized proteins within one or more polymeric nanoparticles for protecting said protein from detrimental environmental conditions.
- View Dependent Claims (15, 16, 17, 18, 19, 20, 21)
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22. A nanoparticle comprising:
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a functionalized enzyme conjugate comprising one or more enzymes or biological catalysts, said enzymes or biological catalysts of said functionalized enzyme conjugate being modified with a hydrophobic chemical group and a polymerizable chemical group; and a thermally responsive polymer, said functionalized enzyme conjugate encapsulated within said thermally responsive polymer. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
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Specification