Hydrophobic Interaction Chromatography Membranes, and Methods of Use Thereof
First Claim
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1. A composite material, comprising:
- a support member, comprising a plurality of pores extending through the support member; and
a macroporous cross-linked gel, comprising a plurality of macropores, and a plurality of pendant hydrophobic moieties;
wherein the macroporous cross-linked gel is located in the pores of the support member; and
the average pore diameter of the macropores is less than the average pore diameter of the pores.
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Abstract
Described herein are composite materials and methods of using them for hydrophobic interaction chromatography (HIC). In certain embodiments, the composite material comprises a support member, comprising a plurality of pores extending through the support member; and a macroporous cross-linked gel, comprising a plurality of macropores, and a plurality of pendant hydrophobic moieties. In certain embodiments, the composite materials may be used in the separation or purification of a biological molecule or biological ion.
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24 Claims
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1. A composite material, comprising:
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a support member, comprising a plurality of pores extending through the support member; and a macroporous cross-linked gel, comprising a plurality of macropores, and a plurality of pendant hydrophobic moieties; wherein the macroporous cross-linked gel is located in the pores of the support member; and
the average pore diameter of the macropores is less than the average pore diameter of the pores. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method of making a composite material, comprising the steps of:
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combining a monomer, a photoinitiator, a cross-linking agent, and a solvent, thereby forming a monomeric mixture; contacting a support member with the monomeric mixture, thereby forming a modified support member;
wherein the support member comprises a plurality of pores extending through the support member, and the average pore diameter of the pores is about 0.1 to about 25 μ
m;covering the modified support member with a polymeric sheet, thereby forming a covered support member; and irradiating the covered support member for a period of time, thereby forming a composite material.
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Specification