METHOD FOR PROLIFERATION OF PLURIPOTENT STEM CELLS
First Claim
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1. A method for proliferation of pluripotent stem cells, which comprises culturing the pluripotent stem cells in a medium free from both feeder cells and serum in a system containing laminin-5.
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Abstract
For efficient proliferation of pluripotent stem cells in a system free from any animal-derived material such as feeder cells or serum, the present invention provides a method for proliferation of pluripotent stem cells, which comprises culturing the pluripotent stem cells in a medium free from both feeder cells and serum in a system containing laminin-5.
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14 Claims
- 1. A method for proliferation of pluripotent stem cells, which comprises culturing the pluripotent stem cells in a medium free from both feeder cells and serum in a system containing laminin-5.
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13. Use of laminin-5 as a cell-supporting material for proliferation of pluripotent stem cells.
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14. A culture kit for pluripotent stem cells, which comprises a laminin-5-treated culture vessel and a serum replacement.
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