METHOD FOR PRODUCTION OF PLURIPOTENT STEM CELL

• US 20110117653A1
• Filed: 07/07/2009
• Published: 05/19/2011
• Est. Priority Date: 07/07/2008
• Status: Active Grant

• Current Assignee: Takara Bio Incorporated (Ningbo Junsheng Investment Group Co., Ltd.)
• Original Assignee: Takara Bio Incorporated (Ningbo Junsheng Investment Group Co., Ltd.)
• Inventors: Iwamoto, Fumiko, Marui, Takahiro, K, Ikunoshin, Nishie, Toshikazu, Enoki, Tatsuji, Takashima, Fuyuko
• Granted Patent: US 9,238,797 B2
• Time in Patent Office: Days
• US Class Current: 435/455
• CPC Class Codes: C12N 2501/405 Cell cycle regulated proteins, e.g. cyclins, cyclin-dependant kinases
• CPC Class Codes: C12N 2501/602 Sox-2
• CPC Class Codes: C12N 2501/603 Oct-3/4
• CPC Class Codes: C12N 2501/604 Klf-4
• CPC Class Codes: C12N 2501/605 Nanog
• CPC Class Codes: C12N 2501/608 Lin28
• CPC Class Codes: C12N 2501/727 Kinases (EC 2.7.)
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2533/52 Fibronectin; Laminin
• CPC Class Codes: C12N 2740/15043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2740/15071 Demonstrated in vivo effect
• CPC Class Codes: C12N 5/0696 Artificially induced pluripotent stem cells, e.g. iPS

Abstract

The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28.

Claims

1-17. -17. (canceled)

18. A method for production of a cell population containing a pluripotent stem cell, said method comprising a step of infecting a somatic cell with a retrovirus(s) vector carrying genes encoding nuclear reprogramming factors in the presence of a functional substance having an activity of binding to a retrovirus.

23. A method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors, under the nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle.

28. A method for production of a cell population containing a pluripotent stem cell, said method comprising the following steps:

(1) a step of contacting nuclear reprogramming factors with a somatic cell, and

(2) a step of treating the somatic cell obtained in (1) under the nutrient-starved condition, and/or treating the somatic cell with an agent capable of arresting cell cycle.