PHOTOINDUCED SIGNAL AMPLIFICATION THROUGH EXTERNALLY SENSITIZED PHOTOFRAGMENTATION IN MASKED PHOTOSENSITIZERS AND PHOTOAMPLIFIED FLUORESCENCE TURN-OFF SYSTEM

• US 20110117667A1
• Filed: 08/20/2008
• Published: 05/19/2011
• Est. Priority Date: 08/20/2007
• Status: Active Grant

• Current Assignee: University of Denver
• Original Assignee: University of Denver
• Inventors: Kutateladze, Andrei G., Majjigapu, Janaki, Kurchan, Alexei, Kottani, Rudresha
• Granted Patent: US 8,735,167 B2
• Time in Patent Office: Days
• US Class Current: 436/172
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

Provided is a photoamplified fluorescence turn-off assay where a masked photosensitizer is mixed with a fluorescent molecule. This mixture is brightly fluorescent because the masked photosensitizer is not capable of quenching the fluorophore. When the photosensitizer is released and amplified, the photosensitizer quenches the emission of fluorophores very efficiently.

Claims

1. A method of amplified fluorescence quenching comprising:

(a) providing a plurality of masked photosensitizers, each masked photosensitizer having a masking group bonded to a quenching photosensitizer through a releasable covalent bond which disrupts the conjugation of the quenching photosensitizer;

(b) providing a fluorophor in quenching proximity to a first masked photosensitizer;

(c) exciting the unmasked photosensitizer with photoradiation, whereby this excited sensitizer induces fragmentation in masked sensitizer, inducing release of the masking group from the first masked photosensitizer, producing a quenching photosensitizer which (1) reduces the fluorescence of the fluorophor and (2) induces the release of the masking group from a second masked photosensitizer; and

(d) repeating step (c).

2. The method of claim 1, wherein the inducing step is performed by exciting the first masked photosensitizer with photoradiation at a cleaving wavelength.

3. The method of claim 1, wherein the inducing step is performed by the reaction of a masked photosensitizer with an unmasked photosensitizer.

4. The method of claim 1, wherein the photosensitizer is selected from the group consisting of:

benzophenone, substituted benzophenone, xanthone, anthraquinone, and any sensitizer which has an excited state capable of oxidizing the masking group.

5. The method of claim 1, wherein the fluorophor is a laser dye.

6. The method of claim 5, wherein the fluorophor is p-terphenyl.

7. The method of claim 1, wherein the fluorophor is a polyphenyl, which includes biphenyl, and quaterphenyl, or any fluorophore not absorbing UV/Vis light in the vicinity of photoamplification wavelength.

8. The method of claim 1, wherein the masking group is a member of the group consisting of:

dithiane, trithiane, dithiazine, tert-alkyl, nitrile, carboxamide, and other radical leaving groups.

9. The method of claim 1, wherein the plurality of masked photosensitizers is attached to a support.

10. The method of claim 9, wherein the support is a dendrimer, particle, surface or liposome.

11. The method of claim 10, wherein the surface is selected from the group consisting of:

conductive, semi-conductive, or non-conductive.

12. The method of claim 1, wherein at least one of the plurality of photosensitizers is attached to a first member of a ligand-receptor pair and the reaction photosensitizer is attached to the second member of a ligand-receptor pair.

13. The method of claim 1, wherein the masked photosensitizers and fluorophors are present in a gelated solvent.

14. The method of claim 13, wherein the gelated solvent is organic.

15. The method of claim 14, wherein the gelated organic solvent is selected from the group consisting of:

alkanes, such as hexane, heptane, octane, nonane, decane and their isomers, benzene, substituted benzenes including tert-butylbenzene, bis-alkylbenzenes, tri-alkylbenzenes, dichlorobenzene and other high boiling point lipophilic solvents.

16. The method of claim 13, wherein the gelated solvent is an ionic liquid.

17. The method of claim 16, wherein the ionic liquid contains one or more hydrophobic alkyl groups.

18. The method of claim 17, wherein the ionic liquid is a tetraalkylammonium or imidazolium salt.

19. An array of pixels comprising:

a plurality of spatially separated groups of masked photosensitizers in a gelated solvent, each group comprising a plurality of masked photosensitizers, each masked photosensitizer having a masking group bonded to a photosensitizer through a releasable covalent bond which disrupts the conjugation of the photosensitizer, said masked photosensitizers in each group in releasing proximity with each other.

20. The array of claim 19, wherein the gelated solvent is organic.

21. The array of claim 19, wherein the gelated solvent is an ionic liquid.