MULTIPLE DISPLACEMENT AMPLIFICATION
First Claim
1. A method comprising:
- contacting a sample of nucleic acid with a reaction mixture comprising a set of oligonucleotide primers, one or more polymerase enzymes and one or more emulsion-forming components; and
subjecting said reaction mixture to conditions under which said nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction.
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Accused Products
Abstract
The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.
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Citations
26 Claims
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1. A method comprising:
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contacting a sample of nucleic acid with a reaction mixture comprising a set of oligonucleotide primers, one or more polymerase enzymes and one or more emulsion-forming components; and subjecting said reaction mixture to conditions under which said nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A kit for performing multiple displacement amplification of a nucleic acid, said kit comprising:
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one or more polymerases; a plurality of primers; and an emulsion-forming component.
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22. A system for analyzing nucleic acids comprising:
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a thermal cycler; a container for holding a reaction mixture, said container configured for insertion into said thermal cycler, said container compatible with one or more detergents or hydrophobic solvents; and one or more liquid handlers configured to deliver a nucleic acid sample to said container; and to deliver an emulsion-breaking compound to said container. - View Dependent Claims (23, 24, 25, 26)
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Specification