METHOD FOR PREPARING NUCLEOTIDES AND RELATED ANALOGUES BY SYNTHESIS ON SOLUBLE SUBSTRATE, AND BIOLOGICAL TOOLS THUS PREPARED
First Claim
1. A method for preparing a modified nucleotide or nucleotide analogue monomer comprising the following steps:
- (1) coupling (i) a soluble polyethylene glycol support with a molecular weight in the range 3000 to 6000 provided with at least one acid, diacid or ether-acid linker and (ii) a nucleoside or nucleoside analogue monomer at an exocyclic amine or hydroxyl group of the nucleoside or nucleoside analogue, using a coupling agent; and
(2) phosphorylating said nucleoside or nucleoside analogue coupled to said support with a phosphorylation agent.
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Abstract
The invention concerns a method for preparing monomer nucleotides or nucleotide analogues comprising the steps of: (1) coupling a soluble polyethylene glycol support provided with at least one diacid or ether-acid linker and a monomer nucleoside or nucleoside analogue to an amine group or hydroxyl group of the nucleoside with the aid of a coupling agent; (2) at least one step for phosphorylation of said nucleoside or nucleoside analogue coupled to said support with a phosphorylation agent; (3) cleavage of said support and recovery of at least one monomer nucleotide or nucleotide analogue. The nucleotides prepared are biological tools.
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28 Claims
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1. A method for preparing a modified nucleotide or nucleotide analogue monomer comprising the following steps:
- (1) coupling (i) a soluble polyethylene glycol support with a molecular weight in the range 3000 to 6000 provided with at least one acid, diacid or ether-acid linker and (ii) a nucleoside or nucleoside analogue monomer at an exocyclic amine or hydroxyl group of the nucleoside or nucleoside analogue, using a coupling agent; and
(2) phosphorylating said nucleoside or nucleoside analogue coupled to said support with a phosphorylation agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
- (1) coupling (i) a soluble polyethylene glycol support with a molecular weight in the range 3000 to 6000 provided with at least one acid, diacid or ether-acid linker and (ii) a nucleoside or nucleoside analogue monomer at an exocyclic amine or hydroxyl group of the nucleoside or nucleoside analogue, using a coupling agent; and
Specification