SYNTHESIS OF OLIGOMERS IN ARRAYS

• US 20110123411A1
• Filed: 11/29/2010
• Published: 05/26/2011
• Est. Priority Date: 06/30/2004
• Status: Abandoned Application

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: BUTLER, John H.
• Application Number:
  US12/955,798
• Publication Number:
  US 20110123411A1
• Time in Patent Office: Days
• US Class Current: 422/238
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/0036 Nozzles
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00414 using suction
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00423 using filtration, e.g. through porous frits
• CPC Class Codes: B01J 2219/00454 by chemical cleavage from the solid support
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides

Abstract

Systems, including apparatus and methods, for synthesis of oligomers in arrays.

Claims

1. A device for parallel synthesis of oligomers, comprising:

a porous member including an array of islands and a spacer joined to the array of islands and separating the islands, the array of islands being more hydrophilic than the spacer, each island defining a plurality of pores permitting reagents to pass through such island;

a channel structure disposed adjacent the porous member and including a plurality of reaction compartments disposed in one-to-one correspondence with the array of islands; and

a set of particles disposed in each reaction compartment and retained therein by the channel structure, the set of particles being configured to support synthesis of at least one oligomer using the reagents that pass through the corresponding island of the array.

2. A device for directing fluids, comprising:

a porous member including an array of islands and a spacer joined to the array of islands and separating the islands, the islands and the spacer having different surface energies, the porous member including opposing surfaces, each island defining a network of pores and being configured to receive reagents and to permit passage of the reagents between the opposing surfaces.