Compositions For Use In Detection Of Multiple Analytes
First Claim
1. A method for determining the presence or relative amounts of two or more components in a medium, said method comprising:
- a) providing in combination (1) a medium suspected of containing said components, (2) at least two sensitizer reagents, said sensitizer reagents being capable of generating a product upon activation, said sensitizer reagents being distinguishable on the basis of activation, and (3) at least one reactive reagent activatable by said product, wherein the combination of sensitizer reagent and reactive reagent allows differential detection of said components, andb) differentially activating said sensitizer reagents and detecting the amount of signal generated corresponding to the activation of said reactive reagent by said product, the amount thereof being related to the amount of each of said components in said medium.
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Accused Products
Abstract
Methods, compositions and kits are disclosed. The methods are directed to determining the presence or relative amounts of two or more components in a medium. A combination is provided comprising a medium suspected of containing the components, at least two sensitizer reagents and at least one reactive reagent activatable by singlet oxygen. The sensitizer reagents are capable of generating singlet oxygen and are distinguishable by wavelength of sensitization. The combination of sensitizer reagents and reactive reagents allows differential detection of the components. The sensitizer reagents are differentially activated. The amount of signal generated as a result of the activation of said reactive reagent is determined wherein the amount thereof is related to the amount of each of the components in the medium.
15 Citations
39 Claims
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1. A method for determining the presence or relative amounts of two or more components in a medium, said method comprising:
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a) providing in combination (1) a medium suspected of containing said components, (2) at least two sensitizer reagents, said sensitizer reagents being capable of generating a product upon activation, said sensitizer reagents being distinguishable on the basis of activation, and (3) at least one reactive reagent activatable by said product, wherein the combination of sensitizer reagent and reactive reagent allows differential detection of said components, and b) differentially activating said sensitizer reagents and detecting the amount of signal generated corresponding to the activation of said reactive reagent by said product, the amount thereof being related to the amount of each of said components in said medium.
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2. A method for determining the presence or relative amounts of two or more components in a medium, said method comprising:
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a) providing in combination (1) a medium suspected of containing said components, (2) at least two sensitizer reagents, said sensitizer reagents being capable of generating singlet oxygen and being distinguishable on the basis of activation, and (3) at least one reactive reagent activatable by singlet oxygen, wherein the combination of sensitizer reagents and reactive reagents allows differential detection of said components, and b) differentially activating said sensitizer reagents and detecting the amount of signal generated as a result of the activation of said reactive reagent, the amount thereof being related to the amount of each of said components in said medium. - View Dependent Claims (3, 4, 5, 6)
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7. A method for determining the presence or relative amounts of two or more components in a medium, said method comprising:
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a) providing in combination (1) a medium suspected of containing said components, (2) at least two sensitizer reagents, said sensitizer reagents being capable of generating singlet oxygen and being distinguishable by wavelength of sensitization, and (3) at least one reactive reagent activatable by singlet oxygen wherein a first specific binding pair (sbp) member is associated with each of said sensitizer reagents and wherein said first sbp member is capable of binding to said component or to a second sbp member to form a complex related to the amount of said component, and b) differentially activating said sensitizer reagents and detecting the amount of signal generated as a result of the activation of each of said reactive reagents, the amount thereof being related to the amount of each of said components in said medium. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20, 21, 22, 23, 24)
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18. A homogeneous method for determining the presence or relative amounts of two or more components in a medium, said method comprising:
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a) providing in combination (1) a medium suspected of containing said components, (2) a photosensitizer for each of said components, said photosensitizer being associated with a first particle and being capable of generating singlet oxygen and having different wavelengths of sensitization, and (3) chemiluminescent compositions activatable by singlet oxygen, each of which is associated with a second particle, wherein one or more of said chemiluminescent compositions are differentially detectable by different wavelengths of emission or by different rates of decay and wherein the different wavelengths of sensitization and the different wavelengths of emission or different rates of decay result in the differential detection of each of said components and wherein a first specific binding pair (sbp) member is associated with each of said photosensitizers and wherein said first sbp member is capable of binding to said component or to a second sbp member to form a complex related to the amount of said component, and b) differentially irradiating said photosensitizer with light and detecting the amount of luminescence generated by each of said chemiluminescent compositions at a time after activation and at a wavelength corresponding to the rate of decay and wavelength of said luminescent emission, the amount thereof being related to the amount of each of said components in said medium.
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25. A kit comprising in packaged combination:
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(a) a plurality of sensitizer reagents, each comprising (1) a sensitizer composition that is capable of generating singlet oxygen and (2) a member of a specific binding pair (sbp), wherein at least a portion of said sensitizer compositions have different wavelengths of sensitization, and b) at least one reactive reagent that is activatable by singlet oxygen. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33)
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- 34. A compound which is bis(tri-alkyl1-silyl)silicon tetra-alkyl2-naphthalocyanine.
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38. A compound which is dihydroxysilicon tetra-t-butyl-naphthalocyanine or dichlorosilicon tetra-t-butyl-naphthalocyanine.
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39. A method of making bis(tri-n-hexylsilyl)silicon tetra-t-butyl-naphthalocyanine, said method comprising treating dihydroxysilicon tetra-t-butyl-naphthalocyanine with tri-n-hexylsilylchloride.
Specification