TRANSGENIC MAIZE EVENT MON 87427 AND THE RELATIVE DEVELOPMENT SCALE

• US 20110126310A1
• Filed: 11/16/2010
• Published: 05/26/2011
• Est. Priority Date: 11/23/2009
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Kelly, Rebecca A., Garnaat, Carl W., Heredia, Oscar, Huang, Jintai, Stoecker, Martin A., Fonseca, Agustin E., Feng, Paul C.C.
• Granted Patent: US 8,618,358 B2
• Time in Patent Office: Days
• US Class Current: 800/275
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8289 Male sterility
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The invention provides transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87427. The invention also provides nucleotides specific for transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products comprising nucleotides specific for transgenic maize event MON 87427. The invention also provides methods related to transgenic maize event MON 87427 and to the Roundup® Hybridization System (RHS). The invention also provides a Relative Development Scale useful for monitoring and determining reproductive development in maize that reconciles developmental differences across various maize varieties. This is useful for determining the optimal timing of a treatment regimen in which tassel development stage is an important factor, including various methods in making hybrid seed.

Claims

1. A recombinant DNA molecule comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:

1-8.

2. The recombinant DNA molecule of claim 1, wherein said recombinant DNA molecule is formed by the junction of an inserted heterologous nucleic acid molecule and genomic DNA of a maize plant, plant cell, or seed.

3. The recombinant DNA molecule of claim 1, wherein said DNA molecule is derived from transgenic maize event MON 87427, a representative sample having been deposited as ATCC PTA-7899.

4. The recombinant DNA molecule of claim 3, wherein said DNA molecule is an amplicon diagnostic for the presence of DNA derived from transgenic maize event MON 87427.

5. The recombinant DNA molecule of claim 1, wherein said DNA molecule is in a maize plant, plant cell, seed, progeny plant, plant part, or commodity product derived from transgenic maize event MON 87427, a representative sample having been deposited as ATCC PTA-7899.

6. A DNA molecule comprising a nucleic acid molecule having a nucleotide sequence of sufficient length of contiguous nucleotide sequence of SEQ ID NO:

10 to function as a DNA probe that hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO;

1-10 and does not hybridize under the stringent hybridization conditions with a DNA molecule not comprising a nucleotide sequence selected from the group consisting of SEQ ID NO;

1-10.

8. A method of detecting the presence of a DNA molecule derived from MON 87427 in a sample, said method comprising:

a. contacting a sample with the DNA probe of claim 6;

b. subjecting said sample and said DNA probe to stringent hybridization conditions; and

c. detecting hybridization of said DNA probe to a DNA molecule in said sample, wherein the hybridization of said DNA probe to said DNA molecule indicates the presence of a DNA molecule derived from transgenic maize event MON 87427 in said sample.

7. A pair of DNA molecules consisting of a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein said first and second DNA molecules each comprise a nucleic acid molecule having a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:

10 to function as DNA primers when used together in an amplification reaction with DNA derived from event MON 87427 to produce an amplicon diagnostic for transgenic maize event MON 87427 DNA in a sample.

9. A method of detecting the presence of a DNA molecule derived from transgenic maize event MON 87427 in a sample, said method comprising:

a. contacting a sample with the pair of DNA molecules of claim 7;

b. performing an amplification reaction sufficient to produce a DNA amplicon comprising a sequence selected from the group consisting of SEQ ID NO;

1-10; and

c. detecting the presence of said DNA amplicon in said reaction, wherein the presence of said DNA amplicon in said reaction indicates the presence of a DNA molecule derived from MON 87427 in said sample.

10. A DNA detection kit comprising at least one DNA molecule comprising a nucleotide sequence of sufficient length of contiguous nucleotide sequence of SEQ ID NO:

10 to function as a DNA primer or probe specific for detecting the presence of DNA derived from transgenic maize event MON 87427, wherein detection of said DNA is diagnostic for the presence of said transgenic maize event MON 87427 DNA in a sample.

11. A recombinant maize plant, seed, cell, or plant part thereof comprising a nucleic acid molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO:

1-10.

12. The recombinant maize plant, seed, cell, or plant part thereof of claim 11, wherein said plant, seed, cell, or plant part thereof has tissue-selective tolerance to glyphosate herbicide treatment.

13. The recombinant maize plant, seed, cell, or plant part thereof of claim 11, the genome of which produces an amplicon comprising a DNA molecule selected from the group consisting of SEQ ID NO:

1-10 when tested in a DNA amplification method.

14. A maize plant or seed, wherein said maize plant or seed is derived from transgenic maize event MON 87427, a representative sample having been deposited under ATCC accession No. PTA-7899.

15. The maize plant or seed of claim 14, wherein said maize plant or seed is a hybrid having at least one parent derived from transgenic maize event MON 87427.

16. A nonliving plant material comprising a recombinant DNA molecule selected from the group consisting of SEQ ID NO:

1-10.

17. A microorganism comprising a nucleic acid molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO:

1-10.

18. The microorganism of claim 17, wherein said microorganism is a plant cell.

19. A commodity product produced from transgenic maize event MON 87427 and comprising a nucleic acid molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO:

1-10, wherein detection of said nucleotide sequence in a sample derived from said commodity product is determinative that said commodity product was produced from said transgenic maize event MON 87427.

21. A method of producing the commodity product of claim 19, said method comprising:

a. obtaining a maize plant or part thereof comprising transgenic maize event MON 87427; and

b. producing a maize commodity product from the maize plant or part thereof.

20. The commodity product of claim 20, wherein said commodity product is selected from the group consisting of whole or processed seeds, animal feed, oil, meal, flour, flakes, bran, biomass, and fuel products.

22. A method for controlling weeds in a field comprising planting MON 87427 plants in a field and applying an effective dose of glyphosate herbicide to control weeds in said field without injuring said transgenic maize event MON 87427 plants.

23. The method of claim 22, wherein said effective dose of glyphosate herbicide is from about 0.1 pound to about 4 pounds per acre.

24. A method of producing a maize plant that tolerates application of glyphosate herbicide comprising:

a. sexually crossing a transgenic maize event MON 87427 plant comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO;

1-10 with a second maize plant, thereby producing seed;

b. collecting said seed produced from said cross;

c. growing said seed to produce a plurality of progeny plants;

d. treating said progeny plants with glyphosate; and

e. selecting a progeny plant that is tolerant to glyphosate.

25. A method of producing a maize plant that tolerates application of glyphosate herbicide comprising:

a. selfing a transgenic maize event MON 87427 plant comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO;

1-10, thereby producing seed;

b. collecting said seed produced from said selfing;

c. growing said seed to produce a plurality of progeny plants;

d. treating said progeny plants with glyphosate; and

e. selecting a progeny plant that is tolerant to glyphosate.

26. A method of producing hybrid maize seed comprising:

a. planting transgenic maize event MON 87427 seed in an area;

b. growing a maize plant from said seed;

c. treating said plant with an effective dose of glyphosate herbicide prior to pollen formation to make said plant male sterile without injuring said plant;

d. fertilizing said plant with pollen from a second parent plant; and

e. harvesting seed from said plant, wherein said seed is hybrid maize seed produced by the cross of transgenic maize event MON 87427 plants with a second parent plant.

27. The method of claim 26, wherein said effective dose of glyphosate herbicide is from about 0.1 pound to about 4 pounds per acre.

28. The method of claim 26, further comprising planting a second parent plant seed in said area and growing a maize plant from said second parent plant.

29. The method of claim 28, wherein said second parent plant is glyphosate tolerant.

30. A method for predicting the timing of maize tassel development comprising selecting a range on a Relative Development Scale, wherein said range indicates maturation to a desired tassel development stage.

31. The method of claim 30, wherein said desired tassel development stage is the optimal tassel development stage for reproductive crossing, tassel sterilization, detasseling, or administration of a development modulating treatment to a maize plant.

32. The method of claim 30, wherein the specific flower development stage used to construct said Relative Development Scale is at pollen shed for about 50 percent of a population of maize plants and wherein said range is about 0.62 and about 0.75 on said Relative Development Scale.

33. The method of claim 30, further comprising administering a development modulating treatment to a maize plant at said desired tassel development stage.

34. A method of producing hybrid maize seed comprising:

a. planting maize seed for a first parent plant in an area;

b. growing said first parent plant from said maize seed;

c. determining the timing of tassel development for said first parent plant by selecting a range that indicates maturation to a desired tassel development stage on a Relative Development Scale;

d. using said determination of the timing of tassel development to timely administer a development modulating treatment to said first parent plant thereby preventing self-fertilization of said first parent plant;

e. administering said development modulating treatment to said first parent plant;

f. fertilizing said first parent plant with pollen from a second parent plant; and

g. harvesting seed from said first parent plant, wherein said seed is hybrid maize seed produced by the cross of said first parent plant with said second parent plant.

35. The hybrid maize seed produced using the method of claim 34.

36. The method of claim 34, wherein said development modulating treatment is glyphosate and said first parent plant has tissue-selective glyphosate tolerance.

37. The method of claim 36, wherein said first parent plant is a transgenic maize event MON 87427 plant.

38. The method of claim 37, wherein said second parent plant is glyphosate tolerant.