COMPOSITION FOR GLUCOSE SENSING COMPRISING OF NANOFIBROUS MEMBRANE AND METHOD FOR MANUFACTURING NON-ENZYMATIC GLUCOSE BIOSENSOR USING THE SAME

• US 20110129593A1
• Filed: 10/24/2008
• Published: 06/02/2011
• Est. Priority Date: 07/29/2008
• Status: Active Grant

• Current Assignee: Kyungpook National University Industry-Academic Cooperation Foundation
• Original Assignee: Kyungpook National University Industry-Academic Cooperation Foundation
• Inventors: Lee, Kwang Pill, Shanmuga, Sundaram Komathi, Anantha, Iyengar Gopalan
• Granted Patent: US 8,673,386 B2
• Time in Patent Office: Days
• US Class Current: 427/2.13
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

Disclosed herein is a composition for glucose sensing obtained by dispersing in a solvent such as acetone a nanofibrous membrane fabricated by electrospinning a mixture containing poly(vinylidene fluoride) and poly(aminophenylboronic acid). Also disclosed is a method of fabricating a non-enzymatic glucose biosensor based on an electrospun nanofibrous membrane by depositing the composition on an electrode.

Claims

1. A composition for glucose sensing, which comprises a material obtained by dispersing in a solvent a nanofibrous membrane fabricated by electrospinning a mixture containing poly(vinylidene fluoride) and poly(aminophenylboronic acid).

2. The composition of claim 1, wherein the poly(vinylidene fluoride) and the poly(aminophenylboronic acid) are contained at a ratio of 90-99 wt %:

10-1 wt %.

3. The composition of claim 1, wherein the solvent additionally comprises an additive.

4. The composition of claim 1, wherein the solvent is one or more selected from the group consisting of hydrochloric acid, sulfuric acid, dimethylformamide, diethyl ether, acetone, chloroform, methanol, isopropyl alcohol, methyl ethyl ketone, tetrahydrofuran, toluene, benzene and xylene

5. A method for fabricating a non-enzymatic glucose biosensor based on a nanofibrous membrane, the method comprising the steps of:

(i) electrospinning a mixture containing poly(vinylidene fluoride) and poly(aminophenylboronic acid) to fabricate an electrospun nanofibrous membrane;

(ii) dispersing in a solvent the electrospun nanofibrous membrane obtained in step (i) to prepare a composition for glucose sensing; and

(iii) depositing on an electrode the glucose sensing composition obtained in step (ii).

6. The method of claim 5, wherein the poly(vinylidene fluoride) and poly(aminophenylboronic acid) in step (i) are contained at a ratio of 90-99 wt %:

10-1 wt %.

7. The method of claim 5, wherein the solvent in step (ii) additionally comprises an additive.

8. The method of claim 5, wherein the solvent in step (ii) is one or more selected from the group consisting of hydrochloric acid, sulfuric acid, dimethylformamide, diethyl ether, acetone, chloroform, methanol, isopropyl alcohol, methyl ethyl ketone, tetrahydrofuran, toluene, benzene and xylene.