Compound Screening Using Chondrocytes
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Abstract
This invention provides a system for obtaining cells of the chondrocyte lineage by differentiating primate pluripotent stem cells. The process involves culturing the cells as a micromass or other aggregate form in a cocktail of differentiation agents that facilitates outgrowth of the desired cell type. Progeny are capable of synthesizing Type II collagen or aggrecan, or other products that are characteristic of the chondrocyte lineage. Chondrocytes and chondrocyte precursor cells obtained according to this disclosure are suitable for use in both research and clinical therapy.
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Citations
34 Claims
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1-15. -15. (canceled)
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16. A method of screening a compound for an effect on a chondrocyte in cell culture, comprising:
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a) contacting the cell culture with the compound; and b) observing a change in the chondrocyte in the cell culture, wherein the cell culture comprises chondrocytes that are the differentiated progeny of a non-malignant in vitro cell culture comprising cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81 and, wherein the chondrocytes are at least 99.6% genetically identical to the cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24)
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25. A method of screening a compound for an effect on a cell in cell culture, comprising:
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a. contacting the cell culture with a compound; and b. observing a change in a collagen type II expressing cells in the cell culture, wherein the cell culture comprises cells that express collagen type II and are the differentiated progeny of a non-malignant in vitro cell culture comprising cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-8 and wherein the cells expressing collagen type II are at least 99.6% genetically identical to the cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81 - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification