MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL, 4-HYDROXYBUTANAL, 4-HYDROXYBUTYRYL-COA, PUTRESCINE AND RELATED COMPOUNDS, AND METHODS RELATED THERETO

• US 20110129899A1
• Filed: 10/13/2010
• Published: 06/02/2011
• Est. Priority Date: 10/13/2009
• Status: Active Grant

• Current Assignee: Genomatica Incorporated
• Original Assignee: Genomatica Incorporated
• Inventors: Trawick, John D., Niu, Wei, Burgard, Anthony P., Haselbeck, Robert
• Granted Patent: US 8,377,666 B2
• Time in Patent Office: Days
• US Class Current: 435/243
• CPC Class Codes: C12P 13/001 Amines; Imines
• CPC Class Codes: C12P 19/40 having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
• CPC Class Codes: C12P 7/18 polyhydric
• CPC Class Codes: C12P 7/24 containing a carbonyl group
• CPC Class Codes: C12P 7/42 Hydroxy-carboxylic acids

Abstract

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

Claims

1. A non-naturally occurring microbial organism, comprising a microbial organism having a 4-hydroxybutanal pathway comprising at least one exogenous nucleic acid encoding a 4-hydroxybutanal pathway enzyme expressed in a sufficient amount to produce 4-hydroxybutanal, said 4-hydroxybutanal pathway comprising succinyl-CoA reductase (aldehyde forming);

4-hydroxybutyrate dehydrogenase; and

4-hydroxybutyrate reductase.

2. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises three exogenous nucleic acids encoding succinyl-CoA reductase (aldehyde forming);

4-hydroxybutyrate dehydrogenase; and

4-hydroxybutyrate reductase.

3-55. -55. (canceled)