MULTIPLEXED MICROARRAY AND METHOD OF FABRICATING THEREOF

• US 20110130308A1
• Filed: 12/01/2010
• Published: 06/02/2011
• Est. Priority Date: 12/02/2009
• Status: Abandoned Application

• Current Assignee: Roche NimbleGen, Inc. (Roche Holding AG)
• Original Assignee: Roche NimbleGen, Inc. (Roche Holding AG)
• Inventors: Luckey, John A., Nuwaysir, Emile
• Application Number:
  US12/958,039
• Publication Number:
  US 20110130308A1
• Time in Patent Office: Days
• US Class Current: 506/17
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/142 Preventing evaporation
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence

Abstract

A multiplexed array and method for fabricating a multiplexed array are disclosed. The multiplexed array includes a hydrophobic barrier formed on a substrate. The hydrophobic barrier includes a plurality of wells in which microarrays are located. A liquid cover slip is positioned to seal each of the wells.

Claims

12. A multiplexed array comprising:

a hydrophobic barrier formed on a substrate, the hydrophobic barrier and substrate cooperating to define a plurality of wells, each well including (i) an opening defined in an upper surface of the hydrophobic barrier and (ii) a microarray positioned therein;

a well-forming cover positioned over the hydrophobic barrier, the hydrophobic barrier and the well-forming cover cooperating to laterally contain liquid samples in each one of the plurality of wells when the liquid samples are deposited therein; and

means for removably sealing each well of the plurality of wells.

1. A multiplexed array comprising:

a substrate;

a hydrophobic barrier formed on the substrate, the hydrophobic barrier defining a plurality of wells, each of the plurality of wells including an opening for access to an interior of the well;

a microarray positioned in each of the plurality of wells and attached to the substrate;

a liquid sample deposited in at least one of the plurality of wells; and

a liquid cover slip positioned in contact with the hydrophobic barrier and over the opening of each well to removably seal the plurality of wells.

2. The multiplexed array of claim 1, wherein the hydrophobic barrier is formed from trityl-T amidite.

3. The multiplexed array of claim 1, wherein the hydrophobic barrier defines at least twenty-four wells.

4. The multiplexed array of claim 3, wherein the hydrophobic barrier defines at least ninety-six wells.

5. The multiplexed array of claim 1, wherein the microarray comprises a deoxyribonucleic acid (DNA) microarray.

6. The multiplexed array of claim 1, wherein the liquid cover slip comprises a mineral oil cover slip.

7. The multiplexed array of claim 1, wherein the liquid cover slip comprises a plurality of liquid cover slips, each one of the plurality of liquid cover slips being positioned over at least one opening of the plurality of wells.

8. The multiplexed array of claim 1, wherein the liquid cover slip comprises a quantity of liquid positioned in a container and a portion of the hydrophobic barrier is positioned in the liquid.

9. The multiplexed array of claim 1, further comprising:

a container, wherein the liquid cover slip is positioned in the container.

10. The multiplexed array of claim 1, wherein the substrate comprises a glass substrate.

11. The multiplexed array of claim 1, wherein the substrate comprises a silicon substrate.

13. The multiplexed array of claim 12, wherein the means for removably sealing each well comprises a liquid cover slip.

14. The multiplexed array of claim 12, wherein the hydrophobic barrier and substrate define at least twenty-four wells.

15. The multiplexed array of claim 14, wherein the hydrophobic barrier and substrate define at least ninety-six wells.

16. The multiplexed array of claim 12, wherein each well of the plurality of wells includes a deoxyribonucleic acid (DNA) microarray positioned therein.

17. The multiplexed array of claim 12, wherein the liquid cover slip comprises a mineral oil cover slip.

18. The multiplexed array of claim 12, wherein the liquid cover slip comprises a plurality of liquid cover slips, each one of the plurality of liquid cover slips being positioned over at least one opening of the plurality of wells.

13-1. The multiplexed array of claim 12, wherein the hydrophobic barrier is formed from trityl-T amidite.

19. A method for fabricating a multiplexed array, the method comprising:

synthesizing a plurality of plurality of probes on a common substrate to form a plurality of microarrays;

forming a hydrophobic barrier on the common substrate, the hydrophobic barrier surrounding each microarray to form a plurality of wells, and

positioning a liquid cover slip on the hydrophobic barrier such that the liquid cover slip covers each of the plurality of wells.

20. The method of claim 19, wherein positioning the liquid cover slip comprises inserting at least a portion of the hydrophobic barrier into a bath of mineral oil.