PEPTIDE REAGENTS AND METHOD FOR INHIBITING AUTOANTIBODY ANTIGEN BINDING
First Claim
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1. A reagent for use in an immunoassay for determining the presence or amount of at least one protein in a test sample, the reagent comprising:
- at least one peptide comprising at least 5 consecutive amino acid residues wherein the peptide is derived from said protein and further wherein said reagent is used to block the interaction between an endogenous antibody and said protein in the test sample.
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Abstract
The present disclosure provides immunoassays and kits for detection or quantification of an protein of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte.
35 Citations
34 Claims
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1. A reagent for use in an immunoassay for determining the presence or amount of at least one protein in a test sample, the reagent comprising:
at least one peptide comprising at least 5 consecutive amino acid residues wherein the peptide is derived from said protein and further wherein said reagent is used to block the interaction between an endogenous antibody and said protein in the test sample. - View Dependent Claims (2, 3, 4, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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5. A reagent for use in an immunoassay for determining the presence or amount of at cardiac troponin I in a test sample, the reagent comprising a peptide having a sequence comprising at least five consecutive amino acid residues from a sequence selected from the group consisting of SSDAAREPRPAPAPI (SEQ ID NO:
- 11), VDEERYDIEAKVTKN (SEQ ID NO;
12), DIEAKVTKNITEIAD (SEQ ID NO;
13), LDLRAHLKQVKKEDT (SEQ ID NO;
14), and ALSGMEGRKKKFES (SEQ ID NO;
15).
- 11), VDEERYDIEAKVTKN (SEQ ID NO;
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6. A method of detecting at least one protein of interest in a test sample, the method comprising the steps of:
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a. preparing a first mixture comprising a test sample suspected of containing at least one protein of interest and at least one reagent, wherein said reagent (1) is at least one peptide comprising at least 5 consecutive amino acid residues derived from said protein that binds to the antibody of interest; and
(2) disrupts the interaction between an endogenous antibody in the test sample and the antigen;b. preparing a second mixture comprising the first mixture and a first specific binding partner, wherein the first specific binding partner comprises an antibody, wherein the antibody binds with the protein of interest to form a first specific binding partner-protein complex; and c. contacting the second mixture with a second specific binding partner, wherein the second specific binding partner comprises an antibody that has been conjugated to a detectable label and further wherein the second specific binding partner binds to the first specific binding partner-protein complex to form a first specific binding partner-protein-second specific binding partner complex; and d. measuring the signal generated by or emitted from the detectable label and detecting the protein of interest in the test sample. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification