METHOD FOR PRODUCING BUTANOL USING EXTRACTIVE FERMENTATION WITH OSMOLYTE ADDITION
First Claim
1. A method for recovering butanol from a fermentation medium, the method comprising:
- a) providing a fermentation medium comprising butanol, water, at least one osmolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the osmolyte concentration of the basal fermentation medium and of an optional fermentable carbon source, and a genetically modified microorganism that produces butanol from at least one fermentable carbon source;
b) contacting the fermentation medium with i) a first water-immiscible organic extractant selected from the group consisting of C12 to C22 fatty alcohols, C12 to C22 fatty acids, esters of C12 to C22 fatty acids, C12 to C22 fatty aldehydes, C12 to C22 fatty amides, and mixtures thereof, and optionally ii) a second water-immiscible organic extractant selected from the group consisting of C7 to C22 fatty alcohols, C7 to C22 fatty acids, esters of C7 to C22 fatty acids, C7 to C22 fatty aldehydes, C7 to C22 fatty amides and mixtures thereof to form a two-phase mixture comprising an aqueous phase and a butanol-containing organic phase; and
c) optionally, recovering the butanol from the butanol-containing organic phase to produce recovered butanol.
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Abstract
A method is provided for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one osmolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the osmolyte concentration of the basal fermentation medium and of an optional fermentable carbon source. The osmolyte may comprise a monosaccharide, a disaccharide, glycerol, sugarcane juice, molasses, polyethylene glycol, dextran, high fructose corn syrup, corn mash, starch, cellulose, and combinations thereof. Also provided is a method and composition for recovering butanol from a fermentation medium.
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Citations
26 Claims
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1. A method for recovering butanol from a fermentation medium, the method comprising:
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a) providing a fermentation medium comprising butanol, water, at least one osmolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the osmolyte concentration of the basal fermentation medium and of an optional fermentable carbon source, and a genetically modified microorganism that produces butanol from at least one fermentable carbon source; b) contacting the fermentation medium with i) a first water-immiscible organic extractant selected from the group consisting of C12 to C22 fatty alcohols, C12 to C22 fatty acids, esters of C12 to C22 fatty acids, C12 to C22 fatty aldehydes, C12 to C22 fatty amides, and mixtures thereof, and optionally ii) a second water-immiscible organic extractant selected from the group consisting of C7 to C22 fatty alcohols, C7 to C22 fatty acids, esters of C7 to C22 fatty acids, C7 to C22 fatty aldehydes, C7 to C22 fatty amides and mixtures thereof to form a two-phase mixture comprising an aqueous phase and a butanol-containing organic phase; and c) optionally, recovering the butanol from the butanol-containing organic phase to produce recovered butanol. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 25)
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19. A method for the production of butanol comprising:
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a) providing a genetically modified microorganism that produces butanol from at least one fermentable carbon source; b) growing the microorganism in a biphasic fermentation medium comprising an aqueous phase and i) a first water-immiscible organic extractant selected from the group consisting of C12 to C22 fatty alcohols, C12 to C22 fatty acids, esters of C12 to C22 fatty acids, C12 to C22 fatty aldehydes, C12 to C22 fatty amides, and mixtures thereof, and optionally ii) a second water-immiscible organic extractant selected from the group consisting of C7 to C22 alcohols, C7 to C22-carboxylic acids, esters of C7 to C22 carboxylic acids, C7 to C22 aldehydes, C7 to C22 amides and mixtures thereof, wherein the biphasic fermentation medium further comprises at least one osmolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the osmolyte concentration of the basal fermentation medium and of an optional fermentable carbon source, for a time sufficient to allow extraction of the butanol into the organic extractant to form a butanol-containing organic phase; c) separating the butanol-containing organic phase from the aqueous phase; and d) optionally, recovering the butanol from the butanol-containing organic phase to produce recovered butanol. - View Dependent Claims (20, 21)
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22. A method for the production of butanol comprising:
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a) providing a genetically modified microorganism that produces butanol from a fermentation medium comprising at least one fermentable carbon source; b) growing the microorganism in a fermentation medium wherein the microorganism produces the butanol into the fermentation medium to produce a butanol-containing fermentation medium; c) adding at least one osmolyte to the fermentation medium to provide the osmolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the osmolyte concentration of the basal fermentation medium and of an optional fermentable carbon source; d) contacting at least a portion of the butanol-containing fermentation medium with i) a first water-immiscible organic extractant selected from the group consisting of C12 to C22 fatty alcohols, C12 to C22 fatty acids, esters of C12 to C22 fatty acids, C12 to C22 fatty aldehydes, C12 to C22 fatty amides and mixtures thereof, and optionally ii) a second water-immiscible organic extractant selected from the group consisting of C7 to C22 alcohols, C7 to C22 carboxylic acids, esters of C7 to C22 carboxylic acids, C7 to C22 aldehydes, C7 to C22 amides and mixtures thereof, to form a two-phase mixture comprising an aqueous phase and a butanol-containing organic phase; e) separating the butanol-containing organic phase from the aqueous phase; f) optionally, recovering the butanol from the butanol-containing organic phase; and g) optionally, returning at least a portion of the aqueous phase to the fermentation medium. - View Dependent Claims (23, 24)
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26. A composition comprising
(a) a fermentation medium comprising butanol, water, at least one osmolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the osmolyte concentration of the basal fermentation medium and of an optional fermentable carbon source, and a genetically modified microorganism that produces butanol from at least one fermentable carbon source; -
b) a first water-immiscible organic extractant selected from the group consisting of C12 to C22 fatty alcohols, C12 to C22 fatty acids, esters of C12 to C22 fatty acids, C12 to C22 fatty aldehydes, C12 to C22 fatty amides and mixtures thereof; and c) optionally a second water-immiscible organic extractant selected from the group consisting of C7 to C22 fatty alcohols, C7 to C22 fatty acids, esters of C7 to C22 fatty acids, C7 to C22 fatty aldehydes, C12 to C22 fatty amides and mixtures thereof; wherein a two-phase mixture comprising an aqueous phase and a butanol-containing organic phase may form and whereby butanol may be separated from the fermentation medium of (a).
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Specification