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BEAD READER

  • US 20110136250A1
  • Filed: 11/29/2010
  • Published: 06/09/2011
  • Est. Priority Date: 11/30/2009
  • Status: Active Grant
First Claim
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1. A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising:

  • a) causing a complex of the analyte to fluorescently or chemically emit a first light, or to release a dye;

    b) measuring an integrated intensity of the first light emitted from the beads in the detection area, or a concentration of the dye released from the beads in the detection area, or both;

    c) causing light to interact with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads;

    d) measuring a second light resulting from the interaction with the beads which does not depend on the analyte; and

    e) determining the normalized quantity of analyte from the integrated intensity of the first light or concentration of the dye or both, and from the measured second light.

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