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First Claim
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1. A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising:
- a) causing a complex of the analyte to fluorescently or chemically emit a first light, or to release a dye;
b) measuring an integrated intensity of the first light emitted from the beads in the detection area, or a concentration of the dye released from the beads in the detection area, or both;
c) causing light to interact with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads;
d) measuring a second light resulting from the interaction with the beads which does not depend on the analyte; and
e) determining the normalized quantity of analyte from the integrated intensity of the first light or concentration of the dye or both, and from the measured second light.
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Abstract
A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising:
- a) causing a complex of the analyte to fluorescently or chemically emit a first light, or to release a dye;
- b) measuring an integrated intensity of the first light emitted from the beads in the detection area, or a concentration of the dye released from the beads in the detection area, or both;
- c) causing light to interact with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads;
- d) measuring a second light resulting from the interaction with the beads which does not depend on the analyte; and
- e) determining the normalized quantity of analyte from the integrated intensity of the first light or concentration of the dye or both, and from the measured second light.
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Citations
27 Claims
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1. A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising:
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a) causing a complex of the analyte to fluorescently or chemically emit a first light, or to release a dye; b) measuring an integrated intensity of the first light emitted from the beads in the detection area, or a concentration of the dye released from the beads in the detection area, or both; c) causing light to interact with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads; d) measuring a second light resulting from the interaction with the beads which does not depend on the analyte; and e) determining the normalized quantity of analyte from the integrated intensity of the first light or concentration of the dye or both, and from the measured second light. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 19)
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15. A system for assaying an analyte adhering to beads, the system comprising:
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a) a detection area where the beads with the analyte adhering to them are held; b) a first detector which measures one or more of an integrated intensity of a fluorescent or chemical emission of light from the beads, or a catalyzed release of a dye from the beads, depending on an amount of analyte adhering to them; c) a light source which produces light that interacts with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads; d) a second detector, the same as or different from the first detector, which measures a light received from the beads as a result of the interaction; e) a controller which uses the measurements of the first and second detectors to determine a normalized quantity of analyte adhering to the beads. - View Dependent Claims (16, 17)
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- 18. A method of concentrating magnetic beads, used for assaying an analyte, in a detection area of a cell, the method comprising vibrating the cell while attracting the beads to the detection area using a magnetic field.
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25. A system for assaying an analyte adhering to beads, the system comprising:
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a) a detection cell with a resting surface for the beads; b) a magnet situated to attract the beads towards a detection area of the resting surface, when the beads are located on the resting surface; c) a vibrator which vibrates the detection cell in a manner that facilitates concentration of the beads in the detection area when the magnet is attracting the beads toward the detection area; d) a first detector which measures one or more of an integrated intensity of a fluorescent or chemical emission of light from the beads in the detection area, or a concentration of a dye released from the beads in the detection area, depending on an amount of analyte adhering to the beads; and e) a controller which uses the measurements of the first detector to determine a quantity of analyte adhering to the beads. - View Dependent Claims (26, 27)
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Specification