CHEMICAL REACTION MONITOR
First Claim
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1. A method for synthesizing a plurality of polymers comprising:
- (a) providing a plurality of wells, each well comprising a polymer;
(b) dispensing a reagent comprising a reaction component to said plurality of wells under conditions sufficient for said reaction component to be incorporated into said polymer;
(c) obtaining an image of said plurality of wells and saving said image data to computer memory;
(d) accessing said image data in computer memory and determining a specific value corresponding to the extent of chemical reaction within each well at the time the image was obtained;
(e) determining whether a difference between a value expected if the chemical reaction is successful and the specific value indicates failure of the chemical reaction within a well; and
(f) repeating steps (a) through (e), wherein said dispensing is to at least one well where failure of the chemical reaction is not indicated, while discontinuing dispensing to one or more wells where failure of the chemical reaction is indicated.
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Abstract
Disclosed herein are systems for monitoring chemical reactions. The systems can comprise a lighting device, a camera device for obtaining an image of the chemical reaction mixtures and an analyzer program to process data obtained from the image. Also disclosed are methods of monitoring the progress of chemical reactions using these systems.
104 Citations
20 Claims
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1. A method for synthesizing a plurality of polymers comprising:
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(a) providing a plurality of wells, each well comprising a polymer; (b) dispensing a reagent comprising a reaction component to said plurality of wells under conditions sufficient for said reaction component to be incorporated into said polymer; (c) obtaining an image of said plurality of wells and saving said image data to computer memory; (d) accessing said image data in computer memory and determining a specific value corresponding to the extent of chemical reaction within each well at the time the image was obtained; (e) determining whether a difference between a value expected if the chemical reaction is successful and the specific value indicates failure of the chemical reaction within a well; and (f) repeating steps (a) through (e), wherein said dispensing is to at least one well where failure of the chemical reaction is not indicated, while discontinuing dispensing to one or more wells where failure of the chemical reaction is indicated. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for synthesizing a plurality of polymers comprising:
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(a) providing a substrate comprising a plurality of fixed locations, each location comprising a polymer associated therewith; (b) dispensing a reagent comprising a reaction component to said plurality of locations under conditions sufficient for incorporation of said reaction component into said polymer; (c) obtaining an image of said plurality of locations; (d) determining a specific value corresponding to the extent of incorporation of the reaction component into the polymer at each location at the time the image was obtained; (e) determining whether a difference between a value expected if incorporation of a reaction component into the polymer is successful and said specific value indicates failure of the polymer synthesis at a location; and (f) repeating steps (a) to (e), wherein said dispensing is to locations where failure of the incorporation of a reaction component into the polymer is not indicated, while discontinuing said dispensing to one or more locations where failure of the incorporation of a reaction component into the polymer is indicated - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification