Devices and methods for imaging particular cells including eosinophils

• US 20110137178A1
• Filed: 10/06/2010
• Published: 06/09/2011
• Est. Priority Date: 10/06/2009
• Status: Abandoned Application

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Kang, Dongkyun, Yoo, Hongki, Tearney, Guillermo J., Bouma, Brett E.
• Application Number:
  US12/899,524
• Publication Number:
  US 20110137178A1
• Time in Patent Office: Days
• US Class Current: 600/476
• CPC Class Codes: A61B 5/0062 Arrangements for scanning
• CPC Class Codes: A61B 5/0068 Confocal scanning
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/0086 using infrared radiation
• CPC Class Codes: A61B 5/037 Measuring oesophageal pressure
• CPC Class Codes: A61B 5/411 Detecting or monitoring allergy or intolerance reactions to an allergenic agent or substance
• CPC Class Codes: A61B 5/4233 oesophagus
• CPC Class Codes: A61B 5/68 Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
• CPC Class Codes: A61B 5/6852 Catheters
• CPC Class Codes: A61B 5/6853 with a balloon
• CPC Class Codes: G02B 21/0064 multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence

Abstract

An exemplary embodiment of apparatus and method according to the present disclosure can be provided. For example, using at least one first arrangement, it is possible to direct at least one first electro-magnetic radiation to at least one portion of tissue within a body. Using at least one second arrangement, it is possible to receive at least one second electro-magnetic radiation provided from the portion, which is based on the first electro-magnetic radiation. Further, using at least one third arrangement, it is possible to differentiate at least one particular cell which is eosinophil, mast cell, basophil, monocyte and/or nutrophil from other cells in the portion based on the second electro-magnetic radiation.

Claims

1. An apparatus comprising:

at least one first arrangement configured to direct at least one first electro-magnetic radiation to at least one portion of tissue within a body;

at least one second arrangement configured to receive at least one second electro-magnetic radiation provided from the at least portion, which is based on the at least one first electro-magnetic radiation; and

at least one third arrangement configured to differentiate at least one particular cell which is at least one of eosinophil, mast cell, basophil, monocyte or nutrophil from other cells in the at least one portion based on the at least one second electro-magnetic radiation.

2. The apparatus according to claim 1, wherein the at least one second electro-magnetic radiation is reflected from the at least one portion.

3. The apparatus according to claim 1, wherein the at least one third arrangement is configured to image the at least one particular cell.

4. The apparatus according to claim 3, wherein the at least one third arrangement is configured to image the at least one particular cell over a region of the tissue that greater than an are of 1 mm2.

5. The apparatus according to claim 3, wherein the at least one third arrangement is configured to image the at least one particular cell in three-dimensions.

6. The apparatus according to claim 1, wherein the at least one third arrangement is configured to image a cross-section of the at least one particular cell.

7. The apparatus according to claim 1, wherein the tissue is a luminal organ.

8. The apparatus according to claim 7, wherein the luminal organ is at least one of an esophagus or a pulmonary airway.

9. The apparatus according to claim 1, wherein at least one of the at least one first arrangement or the at least one second arrangement is provided in a catheter.

10. The apparatus according to claim 9, wherein the catheter is structured to be inserted to reach the tissue transorally or transnasally.

11. The apparatus according to claim 9, wherein the catheter has a cross-sectional diameter of less than 5 mm.

12. The apparatus according to claim 9, wherein the catheter includes a balloon arrangement.

13. The apparatus according to claim 12, wherein the balloon arrangement contains an auto-focusing arrangement which is configured to auto-focus on the at least one portion.

14. The apparatus according to claim 9, wherein the catheter is facilitated in a nasogastric tube.

15. The apparatus according to claim 9, wherein the catheter includes a wound cable.

16. The apparatus according to claim 9, wherein the catheter includes a further arrangement which is configured to measure pressure of the tissue within a body.

17. The apparatus according to claim 9, wherein the catheter has a portion to be inserted into the body which is substantially flexible.

18. The apparatus according to claim 1, wherein the at least one particular cell includes a plurality of particular cells, and the at least third arrangement is further configured to determine a number of the particular cells.

19. The apparatus according to claim 1, wherein the at least one particular cell includes a plurality of particular cells, and wherein the at least third arrangement is configured to automatically count a number of the particular cells.

20. The apparatus according to claim 1, wherein the at least one second arrangement is configured to receive a confocal light.

21. The apparatus according to claim 1, wherein the at least one second arrangement is configured to receive a spectrally encoded confocal light.

22. The apparatus according to claim 1, wherein the at least one second arrangement configured to receive and detect a florescent electro-magnetic radiation.

23. The apparatus according to claim 1, wherein at least one of the first arrangement or the second arrangements include a further arrangement which is configured to spectrally disperse at least one of the first electro-magnetic radiation or the second electro-magnetic radiation, respectively.

24. The apparatus according to claim 1, wherein at least one of the at least one first arrangement or the at least one second arrangement contains at least one optical fiber arrangement which has multiple wave-guiding regions.

25. The apparatus according to claim 24, wherein the at least one optical fiber arrangement includes a double-clad fiber core.

26. The apparatus according to claim 1, wherein the at least one first arrangement is configured to transmit at least one a broadband light or light whose frequency changes over time.

27. The apparatus according to claim 1, wherein the at least one third arrangement is further configured to differentiate at least one of basal layer hyperplasia, abscess, eosinophil degranulation or lamina propria fibrosis from other cells.

28. The apparatus according to claim 1, wherein the at least one particular cell is eosinophil.

29. The apparatus according to claim 1, wherein the at least one third arrangement differentiates the at least one particular cell based on at least one of (a) a strength of a signal from a cytoplasm or (ii) a shape of a nucleus of the at least one particular cell.

30. A method comprising:

at least one of receiving or detecting at least one first electro-magnetic radiation provided from at least one portion of tissue within a body which is based on the at least one second electro-magnetic radiation direct to the at least one portion; and

with a computer arrangement, differentiating at least one particular cell which is at least one of eosinophil, mast cell, basophil, monocyte or nutrophil from other cells in the at least one portion based on the at least one first electro-magnetic radiation.