ELECTRODE OF BIOSENSOR, MANUFACTURING METHOD THEREOF, AND BIOSENSOR THEREOF

• US 20110139491A1
• Filed: 06/04/2010
• Published: 06/16/2011
• Est. Priority Date: 12/15/2009
• Status: Active Grant

• Current Assignee: Aeon Diagnostic Technology Corporation
• Original Assignee: Biosensors Electrode Technology Co., Ltd., Yen Hsiang Chang
• Inventors: CHANG, YEN HSIANG
• Granted Patent: US 8,415,566 B2
• Time in Patent Office: Days
• US Class Current: 174/254
• CPC Class Codes: C23C 18/1651 Two or more layers only obtained by electroless plating
• CPC Class Codes: C25D 5/12 at least one layer being of nickel or chromium
• CPC Class Codes: H05K 1/09 Use of materials for the conductive, e.g. metallic pattern
• CPC Class Codes: H05K 2201/0338 Layered conductor, e.g. layered metal substrate, layered finish layer, layered thin film adhesion layer etched tri-metal structure H05K2201/0361

Abstract

The present invention discloses an electrode of a biosensor, a manufacturing method thereof, and a biosensor thereof. The electrode of the biosensor comprises a flexible insulation layer, a resin layer, a first metal layer, a second metal layer, a third metal layer and a fourth metal layer. The resin layer is disposed between the flexible insulating layer and the first metal layer. The second metal layer is disposed between the first metal layer and the third metal layer, and the fourth metal layer is disposed on the third metal layer. The material of the first metal layer comprises copper foil. The material of the second metal layer comprises palladium. The material of the third metal layer comprises nickel, and the material of the fourth metal layer comprises gold or palladium. The electrode further comprises a biological active substance immobilized on the surface of the plurality of metal layers.

Claims

1. An electrode of a biosensor, comprising:

a flexible insulation layer;

a resin layer disposed on a surface of the flexible insulation layer;

a first metal layer disposed on the resin layer;

a second metal layer disposed on the first metal layer;

a third metal layer disposed on the second metal layer; and

a fourth metal layer disposed on the third metal layer;

wherein a material of the first metal layer comprises copper foil, brass, tombac, phosphorous copper, silver or a combination thereof;

a material of the second metal layer comprises palladium;

a material of the third metal layer comprises nickel; and

a material of the fourth metal layer comprises gold or palladium.

2. The electrode of the biosensor as claimed in claim 1, further comprising a bioactive material immobilized on a surface of the fourth metal layers.

3. The electrode of the biosensor as claimed in claim 2, wherein the bioactive material comprises an enzyme, an antigen or an antibody, and the enzyme comprises glucose oxidase, cholesterol esterase, cholesterol oxidase or uricase.

4. The electrode of the biosensor as claimed in claim 1, wherein a material of the flexible insulation layer comprises polyethylene terephthalate, polymide, polyvinyl chloride, polypropylene or flame retardant 4 (FR4), and resin in the resin layer comprises phosphorus epoxy.

5. The electrode of the biosensor as claimed in claim 1, wherein a thickness of the flexible insulation layer is in a range of 0.05 to 1 mm, and a thickness of the resin layer is in a range of 0.001 to 0.1 mm.

6. The electrode of the biosensor as claimed in claim 1, wherein the first metal layer, the second metal layer, the third metal layer and the fourth metal layer are formed by a plating method, and the plating method comprises a evaporation deposition method, a sputter deposition method, a chemical deposition method, or an electrolytic deposition method.

7. A manufacturing method of an electrode of a biosensor, comprising following steps:

providing a flexible insulation layer;

disposing a resin layer on a surface of the flexible insulation layer;

disposing a first metal layer on a surface of the resin layer;

etching a circuit on the first metal layer via a microetching method; and

disposing a second metal layer, a third metal layer and a fourth metal layer sequentially on a surface of the first metal layer comprising the circuit;

wherein a material of the first metal layer comprises copper foil, brass, tombac, phosphorous copper, silver or a combination thereof;

a material of the second metal layer comprises palladium;

a material of the third metal layer comprises nickel; and

a material of the fourth metal layer comprises gold or the palladium.

8. The manufacturing method as claimed in claim 7, further comprising a step of immobilizing a bioactive material on a surface of the fourth layer, wherein the bioactive material comprises an enzyme, an antigen or an antibody, and the enzyme comprises glucose oxidase, cholesterol esterase, cholesterol oxidase or uricase.

9. The manufacturing method as claimed in claim 7, wherein a material of the flexible insulation layer comprises polyethylene terephthalate, polymide, polyvinyl chloride, polypropylene or flame retardant 4 (FR4), and resin in the resin layer comprises phosphorus epoxy.

10. The manufacturing method as claimed in claim 7, wherein a thickness of the flexible insulation layer is in a range of 0.05 to 1 mm, and a thickness of the resin layer is in a range of 0.001 to 0.1 mm.

11. The manufacturing method as claimed in claim 7, wherein the second metal layer, the third metal layer and the fourth metal layer are sequentially disposed on the first metal layer via a evaporation deposition method, a sputter deposition method, a chemical deposition method, or an electrolytic deposition method.

12. A biosensor, comprising:

an electrode, comprising;

a flexible insulation layer;

a resin layer disposed on a surface of the flexible insulation layer;

a first metal layer disposed on the resin layer;

a second metal layer disposed on the first metal layer;

a third metal layer disposed on the second metal layer;

a fourth metal layer disposed on the third metal layer; and

a bioactive material immobilizing on a surface of the fourth metal layer; and

at least one plate disposed on the electrode, an end of the at least one plate provided with an opening;

wherein a material of the first metal layer comprises copper foil, brass, tombac, phosphorous copper, silver or a combination thereof;

a material of the second metal layer comprises palladium;

a material of the third metal layer comprises nickel; and

a material of the fourth metal layer comprises gold or the palladium.

13. The biosensor as claimed in claim 12, wherein a material of the flexible insulation layer comprises polyethylene terephthalate, polymide, polyvinyl chloride, polypropylene or flame retardant 4 (FR4), and resin in the resin layer comprises phosphorus epoxy.

14. The biosensor as claimed in claim 12, wherein the bioactive material comprises an enzyme, an antigen or an antibody, and the enzyme comprises glucose oxidase, cholesterol esterase, cholesterol oxidase or uricase.

15. The biosensor as claimed in claim 12, wherein a thickness of the flexible insulation layer is in a range of 0.05 to 1 mm, and a thickness of the resin layer is in a range of 0.001 to 0.1 mm.

16. The biosensor as claimed in claim 12, wherein the first metal layer, the second metal layer, the third metal layer and the fourth metal layer are formed by a plating method, and the plating method comprises a evaporation deposition method, a sputter deposition method, a chemical deposition method, or an electrolytic deposition method.