Electroactive Scaffold
First Claim
1. An assembly for tissue engineering and/or stem cell differentiation using electrical and/or mechanical stimuli through bioactive fibers comprising at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application.
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Accused Products
Abstract
A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound.
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Citations
26 Claims
- 1. An assembly for tissue engineering and/or stem cell differentiation using electrical and/or mechanical stimuli through bioactive fibers comprising at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application.
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16. A method of manufacturing an assembly for tissue engineering and/or stem cell differentiation comprising:
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(a) providing a first pre-electroded substrate surface; (b) electrospinning a first substantially aligned polymer fiber layer onto the first surface; (c) providing a second pre-electroded substrate surface; (d) electrospinning a second substantially aligned polymer fiber layer onto the second surface; and
,(e) retaining together the layered surfaces with a clamp and/or an adhesive compound. - View Dependent Claims (17, 18, 19, 20, 21)
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22. A method of cell tissue engineering and/or stem cell differentiation, said method comprising:
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(a) providing an assembly having at least two layers of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application to each layer; (b) seeding the assembly with a sample of cells suspended in cell culture media; (c) incubating for an effective time period; (d) applying an effective voltage to one or more layers; and (e) recovering cells and/or a tissue construct. - View Dependent Claims (23, 24, 25, 26)
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Specification