METHODS AND COMPOSITIONS FOR MODULATING IMMUNOLOGICAL TOLERANCE
First Claim
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1. A method for preparing an isolated mesenchymal stem cell population having a defined antigen expression profile comprising determining an antigen expression profile in an isolated mesenchymal stem cell population, andphysically separating the isolated mesenchymal stem cell population based on antigen expression to generate one or more isolated mesenchymal stem cell populations having defined antigen expression profile.
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Abstract
The invention provides compositions and methods for modulating immune responses using mesenchymal stem cells. The invention further provides methods for inducing tolerance to self antigens using mesenchymal stem cells.
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Citations
43 Claims
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1. A method for preparing an isolated mesenchymal stem cell population having a defined antigen expression profile comprising determining an antigen expression profile in an isolated mesenchymal stem cell population, and
physically separating the isolated mesenchymal stem cell population based on antigen expression to generate one or more isolated mesenchymal stem cell populations having defined antigen expression profile.
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8. A method for preparing a mesenchymal stem cell having a defined antigen profile comprising
expressing an exogenous nucleic acid comprising a peripheral tissue antigen in a mesenchymal stem cell.
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9. (canceled)
- 10. An isolated mesenchymal stem cell population having a defined antigen expression profile.
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13. (canceled)
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14. A method for treating a subject having or at risk of developing an autoimmune disease comprising
administering to a subject in need thereof an isolated mesenchymal stem cell population having a defined antigen expression profile in an effective amount to treat the subject.
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16-17. -17. (canceled)
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18. A method for modulating an immune response comprising
administering to a subject in need thereof an isolated mesenchymal stem cell population having a defined antigen expression profile in an effective amount to treat the subject.
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20-22. -22. (canceled)
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23. A method for treating a subject having or at risk of developing an autoimmune disease comprising
administering to a subject in need thereof a mesenchymal stem cell lysate lipophilic fraction in an effective amount to treat the subject.
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24. A method for treating a subject having or at risk of developing an autoimmune disease comprising
administering to a subject in need thereof a mesenchymal stem cell conditioned media lipophilic fraction in an effective amount to treat the subject.
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25-26. -26. (canceled)
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27. A method for preparing an MSC antigen presenting cell comprising
in vitro contacting a naï - ve antigen presenting cell with a mesenchymal stem cell lysate or conditioned media, and
allowing sufficient time for the naive antigen presenting cell to express on its surface an antigen or fragment thereof from the mesenchymal stem cell lysate or conditioned media, thereby generating an MSC antigen presenting cell. - View Dependent Claims (39)
- ve antigen presenting cell with a mesenchymal stem cell lysate or conditioned media, and
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28-38. -38. (canceled)
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40-41. -41. (canceled)
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42. A method for identifying a candidate mesenchymal stem cell comprising
contacting a mesenchymal stem cell with an antigen-specific activated immune cell, and measuring antigen-specific activity of the antigen-specific activated immune cell prior to and after contact with the mesenchymal stem cell, wherein a reduction in antigen-specific activity as a result of contact with the mesenchymal stem cell identifies a candidate mesenchymal stem cell.
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43-44. -44. (canceled)
Specification