Methods and reagents for treating, preventing and diagnosing bunyavirus infection
First Claim
Patent Images
1. A subunit vaccine composition comprising one or more isolated CAL virus immunogens and a pharmaceutically acceptable vehicle, wherein the one or more immunogens are selected from the group consisting of (a) G1, (b) G2, (c) N, (d) NSm, (e) NSs, (f);
- immunogenic fragments of (b), (c), (d) or (e); and
immunogenic analogs of (a), (b), (c), (d), (e) or (f).
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Accused Products
Abstract
Immunogenic compositions for use in treating, preventing and diagnosing infection caused by the California (CAL) serotype of the genus Bunyavirus, such as La Crosse virus (LACV), are disclosed. Also described are reagents for use in diagnostic assays.
16 Citations
96 Claims
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1. A subunit vaccine composition comprising one or more isolated CAL virus immunogens and a pharmaceutically acceptable vehicle, wherein the one or more immunogens are selected from the group consisting of (a) G1, (b) G2, (c) N, (d) NSm, (e) NSs, (f);
- immunogenic fragments of (b), (c), (d) or (e); and
immunogenic analogs of (a), (b), (c), (d), (e) or (f). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 36, 38, 39, 43, 90, 91, 92)
- immunogenic fragments of (b), (c), (d) or (e); and
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11. An immunogenic composition comprising a CAL virus truncated G1 polypeptide, wherein the truncated G1 polypeptide is truncated at a position between amino acid position 1391 and the C-terminus of the native G1 envelope polypeptide, numbered relative to the G1 polypeptide depicted in
FIGS. 1A-1E .
- 13. An immunogenic composition comprising at least one isolated CAL virus immunogen, wherein said immunogen is produced intracellularly.
- 35. An immunogenic composition comprising an inactivated CAL virus and a pharmaceutically acceptable vehicle.
- 37. An immunogenic composition comprising an attenuated CAL virus and a pharmaceutically acceptable vehicle.
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45. A method of producing an immunogenic composition comprising the steps of
(a) providing an inactivated or attenuated CAL virus; - and
(b) combining said inactivated or attenuated CAL virus with a pharmaceutically acceptable vehicle.
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46. A method of producing a subunit vaccine composition comprising the steps of
(a) providing one or more CAL virus immunogens, wherein the one or more immunogens are selected from the group consisting of (a) G1, (b) G2, (c) N, (d) NSm, (e) NSs, (f); - immunogenic fragments of (b), (c), (d) or (e); and
immunogenic analogs of (a), (b), (c), (d), (e) or (f); and(b) combining said CAL virus immunogen(s) with a pharmaceutically acceptable vehicle.
- immunogenic fragments of (b), (c), (d) or (e); and
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47. A method of producing an immunogenic composition comprising the steps of
(a) providing a CAL virus immunogen, wherein said immunogen is produced intracellularly (b) combining said CAL virus immunogen with a pharmaceutically acceptable vehicle.
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48. A method of producing an immunogenic composition comprising the steps of
(a) providing a CAL virus truncated G1 polypeptide, wherein the truncated G1 polypeptide is truncated at a position between amino acid position 1391 and the C-terminus of the native G1 envelope polypeptide, numbered relative to the G1 polypeptide depicted in FIGS. 1A-1E ;- and
(b) combining said CAL virus truncated G1 polypeptide with a pharmaceutically acceptable vehicle.
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49. A method for isolating an immunogenic CAL virus envelope polypeptide comprising:
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(a) providing a population of mammalian host cells that express said envelope polypeptide intracellularly; (b) recovering s membrane component of the cells; (c) treating the membrane component with a non-ionic detergent, thereby to solubilize the membrane component and release the envelope polypeptide; and (d) isolating the released envelope polypeptide. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56)
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57. A CAL virus truncated G1 polypeptide, wherein the truncated G1 polypeptide is truncated at a position between amino acid position 1391 and the C-terminus of the native G1 envelope polypeptide, numbered relative to the G1 polypeptide depicted in
FIGS. 1A-1E .
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59. An isolated oligonucleotide not more than 60 nucleotides in length comprising:
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(a) a nucleotide sequence of at least 10 contiguous nucleotides from a probe or primer sequence depicted in any of FIG. 5 , 6 or 7;(b) a nucleotide sequence having 90% sequence identity to a nucleotide sequence of (a);
or(c) complements of (a) and (b). - View Dependent Claims (60, 61)
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62. An isolated oligonucleotide selected from the group consisting of:
- (a) the oligonucleotide of SEQ ID NO;
7, (b) the oligonucleotide of SEQ ID NO;
8, (c) the oligonucleotide of SEQ ID NO;
9, (d) the oligonucleotide of SEQ ID NO;
10, (e) the oligonucleotide of SEQ ID NO;
11, (f) the oligonucleotide of SEQ ID NO;
12, (g) the oligonucleotide of SEQ ID NO;
13, (h) the oligonucleotide of SEQ ID NO;
14, (i) the oligonucleotide of SEQ ID NO;
15, (j) SEQ ID NO;
16, complements of (a), (b), (c), (d), (e), (f), (g), (h), (i) or (j), and reverse complements of (a), (b), (c), (d), (e), (f), (g), (h), (i) or (j). - View Dependent Claims (63, 64, 65, 66, 67, 81, 82)
- (a) the oligonucleotide of SEQ ID NO;
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68. A method for detecting CAL virus infection in a biological sample, the method comprising:
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(a) isolating nucleic acid from a biological sample suspected of containing CAL virus RNA, wherein if CAL virus is present, said nucleic acid comprises a target sequence; (b) reacting the CAL virus nucleic acid with a detectably labeled probe sufficiently complementary to and capable of hybridizing with the target sequence, wherein said reacting is done under conditions that provide for the formation of a probe/target sequence complex; and (c) detecting the presence or absence of label as an indication of the presence or absence of the target sequence.
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69. A method for detecting La Crosse virus (LACV) infection in a biological sample, the method comprising:
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(a) isolating nucleic acid from a biological sample suspected of containing LACV RNA, wherein if LACV is present, said nucleic acid comprises a target sequence; (b) reacting the LACV nucleic acid with a detectably labeled probe sufficiently complementary to and capable of selectively hybridizing with the target sequence, wherein said reacting is done under conditions that provide for the formation of a probe/target sequence complex; and (c) detecting the presence or absence of label as an indication of the presence or absence of the target sequence. - View Dependent Claims (70)
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71. A method for detecting CAL virus infection in a biological sample, the method comprising:
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isolating nucleic acids from a biological sample suspected of containing CAL virus; amplifying the nucleic acids using at least two primers wherein (a) each of the primers is not more than about 50 nucleotides in length and each of the primers is sufficiently complementary to a portion of the sense and antisense strands, respectively, of CAL virus isolated nucleic acid, if present, to hybridize therewith; and detecting the presence of the amplified nucleic acids as an indication of the presence or absence of CAL virus in the sample. - View Dependent Claims (72, 73)
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74. A method for detecting La Crosse virus (LACV) infection in a biological sample, the method comprising:
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isolating nucleic acids from a biological sample suspected of containing LACV wherein if LACV is present, said nucleic acid comprises a target sequence; amplifying the nucleic acids using at least two primers wherein (a) each of the primers is not more than about 50 nucleotides in length and each of the primers is sufficiently complementary to a portion of the sense and antisense strands, respectively, of LACV isolated nucleic acid, if present, to hybridize therewith, and further wherein at least one of the primers is capable of selectively hybridizing to the target sequence; and detecting the presence of the amplified nucleic acids as an indication of the presence or absence of LACV in the sample. - View Dependent Claims (75, 76, 77)
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78. A method for detecting La Crosse virus (LACV) infection in a biological sample, the method comprising:
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isolating nucleic acids from a biological sample suspected of containing LACV wherein if LACV is present, said nucleic acid comprises a target sequence; amplifying the nucleic acids using at least two primers wherein (a) each of the primers is not more than about 50 nucleotides in length and each of the primers is sufficiently complementary to a portion of the sense and antisense strands, respectively, of LACV isolated nucleic acid, if present, to hybridize therewith; and detecting the presence of the amplified nucleic acids using at least one detectably labeled probe sufficiently complementary to and capable of hybridizing with the LACV nucleic acid if present, as an indication of the presence or absence of LACV in the sample, wherein at least one of the primers and/or the probe is capable of selectively hybridizing to the target sequence. - View Dependent Claims (79)
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80. A kit for detecting a CAL virus infection in a biological sample, the kit comprising:
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primer oligonucleotides wherein the primer oligonucleotides are not more than about 60 nucleotides in length, wherein each of the primers is sufficiently complementary to a portion of the sense and antisense strands, respectively, to CAL virus nucleic acid to hybridize therewith; and written instructions for identifying the presence of a CAL virus.
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83. A kit for detecting a La Crosse virus (LACV) infection in a biological sample, the kit comprising:
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primer oligonucleotides wherein the primer oligonucleotides are not more than about 60 nucleotides in length, wherein each of the primers is sufficiently complementary to a portion of the sense and antisense strands, respectively, to LACV nucleic acid to hybridize therewith and further wherein at least one of the primers is capable of selectively hybridizing to LACV nucleic acid; and written instructions for identifying the presence of a LACV. - View Dependent Claims (84, 85, 86)
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87. A kit for detecting a La Crosse virus (LACV) infection in a biological sample, the kit comprising:
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primer oligonucleotides wherein the primer oligonucleotides are not more than about 60 nucleotides in length, wherein each of the primers is sufficiently complementary to a portion of the sense and antisense strands, respectively, to LACV nucleic acid to hybridize therewith; at least one detectably labeled probe oligonucleotide of not more than about 60 nucleotides in length and sufficiently complementary to and capable of hybridizing with LACV nucleic acid, wherein at least one of the primers and/or the probe is capable of selectively hybridizing to the target sequence; and written instructions for identifying the presence of LACV. - View Dependent Claims (88, 89)
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Specification