BIODEGRADATION PROCESS AND COMPOSITION

• US 20110151508A1
• Filed: 12/21/2010
• Published: 06/23/2011
• Est. Priority Date: 12/23/2009
• Status: Active Grant

• Current Assignee: AMVAC Chemical Corporation (American Vanguard Corporation)
• Original Assignee: Agrinos AS (American Vanguard Corporation)
• Inventors: López-Cervantes, Jaime, Sánchez-Machado, Dalia Isabel, Rochin, Karl Reiner Fick
• Granted Patent: US 8,748,124 B2
• Time in Patent Office: Days
• US Class Current: 435/42
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12R 2001/00 Microorganisms ; Processes using microorganisms

Abstract

Disclosed are novel microbial compositions and biodegradation processes to treat marine animal or marine animal by-products to produce solid, liquid and lipid fractions that contain useful compounds.

Claims

1. A microbial composition comprising ATCC Patent Deposit Designation PTA-10861

9. A biodegradation process comprising:

mixing a marine animal or marine animal by-product with the microbial composition of claim 1 or 2 to form a mixture;

fermenting said mixture; and

separating said mixture into solid, aqueous and lipid fractions.

10. The process of claim 9 wherein said marine animal is a marine arthropod.

11. The process of claim 10 wherein said marine arthropod is selected from the group consisting of shrimp, crab and krill.

12. The process of claim 9 wherein said marine animal is fish.

13. The process of claim 9 wherein said microbial composition comprises ATCC Patent Deposit Designation PTA-10861.

14. The process of claim 9 wherein said fermenting is by facultative aerobic fermentation.

15. The process of claim 9 wherein said separating is by centrifugation.

16. The process of claim 9 wherein said fermenting is at a temperature of about 30°

C. to 40°

C.

17. The process of claim 16 wherein said fermenting is carried out at a pH between about 4.3 and 5.0

18. The process of claim 16 wherein said fermentation is carried out for about 24-96 hours.

19. The process of claim 9 wherein said aqueous fraction comprises amino acids, chitosan and glucosamine

20. The process of claim 19 wherein said aqueous fraction further comprises trace elements.

21. The aqueous fraction made according to claim 9.

22. The process of claim 9 wherein said solid fraction comprises chitin.

23. The solid fraction made according to claim 22.

2. A microbial composition comprising (a) one or more lactic acid bacteria (LAB) and (b) one or more microorganisms selected from the group of genera consisting of Bacillus, Azotobacter, Trichoderma, Rhizobium, Clostridium, Pseudomonas, Streptomyces, Micrococcus, Nitrobacter and Proteus.

3. The microbial composition of claim 2 wherein said LAB is selected from the genera consisting of Lactobacillus, Pediococcus, Lactococcus, and Streptococcus.

4. The microbial composition of claim 2 wherein said LAB is selected from the group consisting of Lactobacillus acidophilus and Lactobacillus casei.

5. The microbial composition of claim 2 wherein said LAB is selected from the group consisting of Lactobacillus acidophilus (Bioderpac, 2008) and Lactobacillus casei (Bioderpac, 2008).

6. The microbial composition of claim 2 wherein said Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus cereus, Bacillus megateriu, Bacillus licheniformis and Bacillus thuringiensis, said Azotobacter is Azotobacter vinelandii, said Trichoderma is Trichoderma harzianum, said Rhizobium is Rhizobium japonicum, said Clostridium is Clostridium pasteurianu and said Pseudomonas is Pseudomonas fluorescens.

7. The microbial composition of claim 2 wherein said Bacillus is selected from the group consisting of Bacillus subtilis (SILoSil®

BS), Bacillus cereus (Bioderpac,

     2008), Bacillus megaterium (Bioderpac,

     2008), Bacillus licheniformis(Bioderpac,

     2008) and Bacillus thuringiensis (strains HD-1 and HD-73 (SILoSil®

BT)), said Azotobacter is Azotobacter vinelandii (Bioderpac,

     2008), said Trichoderma is Trichoderma harzianum (TRICHOSIL), said Rhizobium is Rhizobium japonicum (Bioderpac,

     2008), said Clostridium is Clostridium pasteurianu (Bioderpac,

     2008) and said Pseudomonas is Pseudomonas fluorescens (Bioderpac,

     2008).

8. The microbial composition of claim 2 wherein at least one of said Bacillus, Azotobacter, Trichoderma, Rhizobium, Clostridium, Pseudomonas, Streptomyces, Micrococcus, Nitrobacter and Proteus is a chitinolytic strain.

24. A composition comprising HYTb.

25. A composition comprising HYTc.